Effect of eIF4E on Autophagy of CD138 Cells in Multiple Myeloma.
10.19746/j.cnki.issn.1009-2137.2019.05.029
- Author:
Meng ZHANG
1
,
2
;
Yan-Ping MA
1
,
3
Author Information
1. Shanxi Medical University
2. Taiyuan 030000, Shanxi Province，China,Department of Hematology,The Second Affiliated Hospital of Shanxi Medical University,Taiyuan 030000, Shanxi Province，China.
3. Taiyuan 030000, Shanxi Province，China,Department of Hematology,The Second Affiliated Hospital of Shanxi Medical University,Taiyuan 030000, Shanxi Province，China,E-mail：myanp18@163.com.
- Publication Type:Journal Article
- MeSH:
Autophagy;
Beclin-1;
Cell Line, Tumor;
Eukaryotic Initiation Factor-4E;
Humans;
Multiple Myeloma
- From:
Journal of Experimental Hematology
2019;27(5):1556-1560
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of eukaryotic translation initiation factor 4E(eIF4E) on the autophagy of CD138 plasma cells in multiple myeloma(MM).
METHODS:Multiple myeloma CD138 plasma cells were treated with eIF4E inhibitor 4EGI, the changes of autophagy-related factors LC3-II and Beclin1 were detected by fluorescent quantitative PCR and Western blot, the changes of cell proliferation inhibition were detected by MTT assay, and cell apoptosis was detected by flow cytometry.
RESULTS:Quantitative fluorescence PCR showed that after treatment of myeloma cells with 4EGI, the expression levels of LC3-II and Beclin1 mRNA gradually increased with the enhancomer of 4EGI concentration and the prolongation of action time, and the differences were statistically significant (48 h: LC3-Ⅱ,r=0.942, Beclin1,r=0.952; 80 μg/ml: LC3-Ⅱ,r=0.966, Beclin1,r=0.998）; Western blot showed that with the enhancement of 4EGI concentration, the expression of LC3-II and Beclin1 protein gradually increased（LC3-Ⅱ,r=0.923, Beclin1,r=0.977）; CCK-8 showed that the inhibition rate of cells gradually increased （r=0.996）; the apoptotic rate of 4EGI-treated groups (23.23±4.47, 7.59±1.67, 2.03±0.19) was significantly different from that of control group (0.03±0.04) (P<0.05).
CONCLUSION:The inhibition of eIF4E can activate the autophagy of CD138 plasma cells in multiple myeloma and induce the death of myeloma cells.
