Relationship between TET2 Gene SNP rs3733609 C/T and JAK2V617F Allele Burden in Patients with Myeloproliferative Neoplasms.
10.19746/j.cnki.issn.1009-2137.2019.05.032
- Author:
Xiang XIAO
1
;
Xiao-Liu LIU
2
;
Xiao-Hui SHEN
3
;
Ming-Yang DENG
4
;
Su-Fang LIU
5
;
Guang-Sen ZHANG
4
Author Information
1. Department of Hematology, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,Department of Rehabilitation, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China.
2. Department of Hematology and Oncology, The Fourth Hospital of Changsha, Changsha 410006, Hunan Province, China.
3. Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450003, Henan Province, China.
4. Department of Hematology, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China.
5. Department of Hematology, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China,E-mail: sufang-liu@csu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Alleles;
DNA-Binding Proteins;
genetics;
Exons;
Humans;
Janus Kinase 2;
genetics;
Mutation;
Myeloproliferative Disorders;
genetics;
Neoplasms;
Proto-Oncogene Proteins;
genetics
- From:
Journal of Experimental Hematology
2019;27(5):1574-1579
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the relationship between the polymorphism of TET2 gene SNP rs3733609 and JAK2V617F allele burden in patients with myeloproliferative neoplasms (MPN).
METHODS:The exon 9 of TET2 gene was amplified by RT-PCR, and the nucleotide sequence of SNP rs3733609 site was analyzed by gene sequencing. The MGB Taqman probe PCR method was used to detect the JAK2V617F allele burden. The correlation of TET2 gene SNP rs3733609 C/T with the JAK2V617F allele burden and clinical parameters was analyzed.
RESULTS:TET2 gene rs3733609 C/T heterozygosity (normal T/T) could be detected in 19 cases of 85 cases of JAK2V617F positive MPN (22.4%) patients, while the TET2 gene rs3733609 C/T heterozygosity could be detected only in 9 of the 106 healthy volunteers, and the incidence was only 8.5% (9/106). Compared with the negative group (TET2 rs3733609 T/T), there was no significant difference in the median age, hemoglobin level and platelet count in the patients with TET2 gene SNP rs3733609 (CT/TC) positive, but the WBC count of peripheral blood and JAK2V617F allele burden significantly increased. In JAK2V617F high allele burden group, TET2 gene SNP rs3733609 was positive in 7 cases (36.8%, 7/19), the ratio was higher than that in the low allele burden group(18.2%, 12/66).
CONCLUSION:TET2 SNP rs3733609 C/T may be a new susceptible allelee, which affects the clinical characteristics and clonal evolution of MPN patients.
