Effect of Quercetin on Apoptosis of Platelets and Its Mechanism.
10.19746/j.cnki.issn.1009-2137.2019.05.039
- Author:
Qian XIAO
1
;
Xiong-Yan CHEN
2
;
Qing OUYANG
3
;
Li-Xing JIANG
4
;
Yi-Qian WU
2
;
Yan-Fang JIANG
2
Author Information
1. Department of Transfusion, Guilin Municipal People's Hospital, Guilin 541002, Guangxi Zhuang Autonomous Region, China,E-mail: xiaoqian6623@126.com.
2. Department of Transfusion, Guilin Municipal People's Hospital, Guilin 541002, Guangxi Zhuang Autonomous Region, China.
3. Department of Laboratorial Medicine, The First Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region , China.
4. Central Blood Stations of Guilin, Guilin 530001, Guangxi Zhuang Autonomous Region , China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Apoptosis Regulatory Proteins;
Blood Platelets;
Quercetin
- From:
Journal of Experimental Hematology
2019;27(5):1612-1616
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of quercetin on the apoptosis of platelets and to analyze the intrinsic mechanism.
METHODS:Firstly, the effects of quecetin on the apoptosis of platelets was detected by flow cytometry. Secondly, Western blot was used to detect the expression of apoptosis-related proteins in the platelets treated with quercetin for 2 and 4 day.
RESULTS:By flow cytometry, it was found that the apoptosis of platelets in the quercetin-treated group (2, 4 and 8 μmol/L) was inhibited, the apoptosis rate of platelets in 2, 4 and 8 μmol/L quercetin group was 3.12%±0.32%, 2.89%±0.15% and 2.31%±0.28%, respectively, which were signigicantly lover than that in control group (P＜0.01). With the increase of quecetin concentration, the proportion ratio of platelets significantly decreased in a concentration-dependent manner（r=-0.9985）. Similar results were observed on the 4th day. Western blot showed that the treatment with quercetin (2, 4 and 8 μmol/L) promoted the expression of anti-apoptotic protein BCL-2, inhibited the expression of pro-apoptotic protein BAX, resulting in a significant increase in the ratio of BCL-2/BAX (P＜0.01), thereby inhibiting the apoptosis of platelets. Similar results were observed on the 4th day.
CONCLUSION:Quercetin can inhibit platelet apoptosis by increasing the ratio of apoptosis-related protein BCL-2/BAX in a concentration-dependent manner.
