Effects of Triptolide on Tc and Th Cell Excursion in Peripheral Blood of Nude Mice with Systemic Lupus Erythematosus BALB/c-un.
10.19746/j.cnki.issn.1009-2137.2019.05.051
- Author:
Yu-Fang LIU
1
;
Hua-Qiong HE
2
;
Yan-Li DING
2
;
Shen-Ying WU
2
;
De-Sen CHEN
2
;
Cai-Ling E
3
Author Information
1. Department of Dermatology, Shiyan Municipal People's Hospital (Affiliated People's Hospital of Hubei Medical and Pharmaceutical College), Shiyan 442000, Hubei Province, China.
2. Basic Medical School, Hubei Medical and Pharmaceutial College, Shiyan 442000, Hubei Province, China.
3. Basic Medical School, Hubei Medical and Pharmaceutial College, Shiyan 442000, Hubei Province, China E-mail: 838054166@qq.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Diterpenes;
Epoxy Compounds;
Female;
Lupus Erythematosus, Systemic;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Phenanthrenes;
T-Lymphocytes, Helper-Inducer
- From:
Journal of Experimental Hematology
2019;27(5):1691-1695
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of triptolide on the excursion of Tc and Th cells in peripheral blood of systemic lupus erythematosus (SLE) BALB/c-un nude mice induced by pristane.
METHODS:Eighteen female BALB/c-un nude mice were randomly divided into blank, SLE and triptolide group, each with 6 mice by random table method. Group SLE and group triptolide were established by single intraperitoneal injection of pristane, and blank group was used as blank control group. SLE model was established by single intraperitoneal injection. Triptolide group was fed with triptolide at the dose of 5 mg/(kg·d), and the blank group and SLE group were fed normally. Blood samples were collected from the caudal vein before treatment and 1, 3 and 6 months after treatment respectively. Fluorescence labeled flow cytometry was used to delect Tc and Th lymphocyte subsets at different stages of treatment.
RESULTS:After treatment for 3 and 6 moths, the percentages of Tcl, Thl cells and CD8, Tcl/Tc2, Thl/Th2 and CD4/CD8 all decreased in the group of triptolide, and the percentage of CD4, Tc2 and Th2 cells increased (P＜0.05).
CONCLUSION:The mechanism of triptolide in the treatment of SLE may be related with the excursion of Tc and Th cells to Tcl and Tc2 to maintain the relative homeostasis of Tc and Th cells at different stage, thus affecting the immune response and the inflammatory reaction.
