MRTF-A Regulates the Proliferation and Migration of Non-small Cell Lung Cancer Cells of A549 through HOTAIR.
10.3779/j.issn.1009-3419.2019.02.02
- Author:
Kun ZHANG
1
;
Yubin ZHOU
1
;
Gang FENG
1
;
Fuchun ZENG
1
Author Information
1. Department of Thoracic Surgery, Sichuan Provincial People's Hospital, Chengdu 610072, China.
- Publication Type:Journal Article
- Keywords:
A549;
HOTAIR;
Lung neoplasms;
MRTF-A;
Migration;
Proliferation
- MeSH:
A549 Cells;
Carcinoma, Non-Small-Cell Lung;
genetics;
metabolism;
physiopathology;
Cell Movement;
Cell Proliferation;
Gene Expression Regulation, Neoplastic;
Humans;
Promoter Regions, Genetic;
RNA, Long Noncoding;
genetics;
metabolism;
Trans-Activators;
genetics;
metabolism
- From:
Chinese Journal of Lung Cancer
2019;22(2):82-89
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Non-small cell lung cancer (NSCLC) is a kind of lung cancer, because its high incidence has been concerned. Therefore, it has great significance to reveal the pathogenesis of NSCLC. As a transcriptional regulatory factor, MATF-A plays an important role in the development of multiple tumors, can regulate the migration process of a variety of tumor cells. HOTAIR is a long non-coding RNA (LncRNA) found in recent years, which expresses abnormally in multiple tumors and is involved in the proliferation and migration of multiple tumors. The aim of this study is to explore the role of MRTF-A through HOTAIR to regulate the proliferation and migration of NSCLC cell A549 cell.
METHODS:We constructed the overexpression plasmid and interfering plasmid of MRTF-A, and detected the effect of MRTF-A on the proliferation and migration of A549 cells by CCK8 and wound healing methods respectively. Then, we designed the siRNA of HOTAIR to detect its effect on the proliferation and migration of A549 cells. Through qRT-PCR, we detected the effect of MRTF-A on HOTAIR expression. Finally, we constructed HOTAIR's promoter, and detect the effect of MRTF-A on HOTAIR promoter activity by luciferase reporter gene test.
RESULTS:Overexpression of MRTF-A promotes the proliferation and migration of A549 cells, while silent MRTF-A inhibits its proliferation and migration. Next, we found that interfered HOTAIR expression inhibited the proliferation of A549 cells. We found that MRTF-A could influence the expression of HOTAIR and regulate the activity of HOTAIR promoter.
CONCLUSIONS:MRTF-A regulates the proliferation and migration of A549 cell through HOTAIR.
