Cyclic RNA Molecule circ_0007766 Promotes the Proliferation of Lung Adenocarcinoma Cells by Up-regulating the Expression of Cyclin D1/CyclinE1/CDK4.
10.3779/j.issn.1009-3419.2019.05.03
- Author:
Shuai ZHANG
1
;
Wenjia XIA
1
;
Gaochao DONG
1
;
Weizhang XU
1
;
Ming LI
1
;
Lin XU
1
Author Information
1. Department of Thoracic Surgery, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing 210009, China.
- Publication Type:Journal Article
- Keywords:
Lung adenocarcinoma;
circRNA;
circ_0007766
- MeSH:
Adenocarcinoma of Lung;
pathology;
Apoptosis;
genetics;
Cell Cycle;
genetics;
Cell Line, Tumor;
Cell Proliferation;
genetics;
Cell-Free Nucleic Acids;
genetics;
Cyclin D1;
genetics;
Cyclin E;
genetics;
Cyclin-Dependent Kinase 4;
genetics;
Humans;
Oncogene Proteins;
genetics;
Up-Regulation;
genetics
- From:
Chinese Journal of Lung Cancer
2019;22(5):271-279
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Cyclic RNA (circRNA) is a new type of non-coding RNA (ncRNA) which is different from traditional linear RNA. More and more studies suggest that circRNA can be used as a biological marker of many malignant tumors and becomes a potential target for treatment. Therefore, searching for new molecular targets of lung adenocarcinoma from the circRNA will help to reveal the new mechanism of the occurrence and development of lung adenocarcinoma, and provide new ideas for clinical diagnosis and treatment. In this study, the biological function of circ_0007766, a highly expressed circRNA found in a screen of lung adenocarcinoma tissue, was verified and analyzed in vitro, so as to preliminarily explore the mechanism of circ_0007766 in promoting the proliferation of lung adenocarcinoma.
METHODS:The expression level of circ_0007766 in lung adenocarcinoma cells was detected by qPCR. Then siRNA was used to knock down the expression of circ_0007766. The effects of knockdown of circ_0007766 on proliferation, cell cycle and apoptosis of lung adenocarcinoma cells were detected by CCK8, scratch test, PI staining and Annexin V/PI double staining. In addition, the biological mechanism of circ_0007766 in lung adenocarcinoma was preliminarily studied by qPCR and Western blots.
RESULTS:The expression of circ_0007766 in lung adenocarcinoma cell lines was detected by qPCR. The expression of circ_0007766 was interfered in SPCA-1 cells. The proliferation and migration abilities of cells were inhibited. The cell cycle was arrested in G0/G1 phase, but the apoptosis was not affected. The deletion of circ_0007766 did not affect the expression of ERBB2, but influenced the mRNA and protein expression of Cyclin D1/Cyclin E1/CDK4.
CONCLUSIONS:In vitro functional studies have shown that circ_0007766 may promote the proliferation and migration of lung adenocarcinoma cells. Further molecular mechanism studies have found that circ_0007766 can up-regulate the expression of Cyclin D1/Cyclin E1/CDK4, which are the key proteins of cell cycle, and thus promote the malignant proliferation of lung adenocarcinoma. From the perspective of circRNA, this study will provide new clues for the pathogenesis, development and prognosis of lung adenocarcinoma, and provide new target for clinical treatment.
