GABA Receptor Activity Suppresses the Transition from Inter-ictal to Ictal Epileptiform Discharges in Juvenile Mouse Hippocampus.
10.1007/s12264-018-0273-z
- Author:
Yan-Yan CHANG
1
;
Xin-Wei GONG
2
;
Hai-Qing GONG
2
;
Pei-Ji LIANG
2
;
Pu-Ming ZHANG
3
;
Qin-Chi LU
4
Author Information
1. Department of Neurology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
2. School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
3. School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China. pmzhang@sjtu.edu.cn.
4. Department of Neurology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China. qinchilu@yahoo.com.
- Publication Type:Journal Article
- Keywords:
Bicuculline;
Epileptiform discharge;
Gamma-aminobutyric acid;
Hippocampal slice;
Micro-electrode array;
Muscimol
- MeSH:
Animals;
Animals, Newborn;
Bicuculline;
pharmacology;
Disease Models, Animal;
Epilepsy;
pathology;
GABA-A Receptor Agonists;
pharmacology;
GABA-A Receptor Antagonists;
therapeutic use;
Hippocampus;
drug effects;
metabolism;
physiopathology;
In Vitro Techniques;
Magnesium;
metabolism;
pharmacology;
Male;
Membrane Potentials;
drug effects;
Mice;
Mice, Inbred C57BL;
Muscimol;
pharmacology;
Nerve Net;
drug effects;
Receptors, GABA-A;
metabolism
- From:
Neuroscience Bulletin
2018;34(6):1007-1016
- CountryChina
- Language:English
-
Abstract:
Exploring the transition from inter-ictal to ictal epileptiform discharges (IDs) and how GABA receptor-mediated action affects the onset of IDs will enrich our understanding of epileptogenesis and epilepsy treatment. We used Mg-free artificial cerebrospinal fluid (ACSF) to induce epileptiform discharges in juvenile mouse hippocampal slices and used a micro-electrode array to record the discharges. After the slices were exposed to Mg-free ACSF for 10 min-20 min, synchronous recurrent seizure-like events were recorded across the slices, and each event evolved from inter-ictal epileptiform discharges (IIDs) to pre-ictal epileptiform discharges (PIDs), and then to IDs. During the transition from IIDs to PIDs, the duration of discharges increased and the inter-discharge interval decreased. After adding 3 μmol/L of the GABA receptor agonist muscimol, PIDs and IDs disappeared, and IIDs remained. Further, the application of 10 μmol/L muscimol abolished all the epileptiform discharges. When the GABA receptor antagonist bicuculline was applied at 10 μmol/L, IIDs and PIDs disappeared, and IDs remained at decreased intervals. These results indicated that there are dynamic changes in the hippocampal network preceding the onset of IDs, and GABA receptor activity suppresses the transition from IIDs to IDs in juvenile mouse hippocampus.