Laminar Distribution of Neurochemically-Identified Interneurons and Cellular Co-expression of Molecular Markers in Epileptic Human Cortex.
10.1007/s12264-018-0275-x
- Author:
Qiyu ZHU
1
;
Wei KE
2
;
Quansheng HE
2
;
Xiongfei WANG
3
;
Rui ZHENG
2
;
Tianfu LI
3
;
Guoming LUAN
3
;
Yue-Sheng LONG
4
;
Wei-Ping LIAO
4
;
Yousheng SHU
5
Author Information
1. College of Pharmaceutical Sciences, Brain Institute, Capital Medical University, Beijing, 100069, China.
2. State Key Laboratory of Cognitive Neuroscience and Learning and IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, 100875, China.
3. Department of Neurosurgery, Epilepsy Center, Sanbo Brain Hospital of Capital Medical University, Beijing Key Laboratory of Epilepsy, Epilepsy Institution, Beijing Institute for Brain Disorders, Beijing, 100093, China.
4. Institute of Neuroscience and Department of Neurology of the Second Affiliated Hospital of Guangzhou Medical University, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Guangzhou, 501260, China.
5. State Key Laboratory of Cognitive Neuroscience and Learning and IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, 100875, China. yousheng@bnu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Cell type;
Cholecystokinin;
Epilepsy;
Human cortex;
Immunostaining;
Interneuron;
Neuropeptide Y;
Parvalbumin;
Somatostatin;
Tyrosine hydroxylase
- MeSH:
Adolescent;
Adult;
Brain Chemistry;
genetics;
physiology;
Cerebral Cortex;
metabolism;
pathology;
Child;
Cholecystokinin;
metabolism;
Epilepsy;
etiology;
pathology;
Female;
Gene Expression Regulation;
physiology;
Humans;
Interneurons;
metabolism;
Male;
Middle Aged;
Neuropeptide Y;
metabolism;
Parvalbumins;
metabolism;
Phosphopyruvate Hydratase;
metabolism;
Somatostatin;
metabolism;
Tyrosine 3-Monooxygenase;
metabolism;
Young Adult
- From:
Neuroscience Bulletin
2018;34(6):992-1006
- CountryChina
- Language:English
-
Abstract:
Inhibitory GABAergic interneurons are fundamental elements of cortical circuits and play critical roles in shaping network activity. Dysfunction of interneurons can lead to various brain disorders, including epilepsy, schizophrenia, and anxiety. Based on the electrophysiological properties, cell morphology, and molecular identity, interneurons could be classified into various subgroups. In this study, we investigated the density and laminar distribution of different interneuron types and the co-expression of molecular markers in epileptic human cortex. We found that parvalbumin (PV) and somatostatin (SST) neurons were distributed in all cortical layers except layer I, while tyrosine hydroxylase (TH) and neuropeptide Y (NPY) were abundant in the deep layers and white matter. Cholecystokinin (CCK) neurons showed a high density in layers IV and VI. Neurons with these markers constituted ~7.2% (PV), 2.6% (SST), 0.5% (TH), 0.5% (NPY), and 4.4% (CCK) of the gray-matter neuron population. Double- and triple-labeling revealed that NPY neurons were also SST-immunoreactive (97.7%), and TH neurons were more likely to express SST (34.2%) than PV (14.6%). A subpopulation of CCK neurons (28.0%) also expressed PV, but none contained SST. Together, these results revealed the density and distribution patterns of different interneuron populations and the overlap between molecular markers in epileptic human cortex.
