Quantification of Tyrosine Hydroxylase and ErbB4 in the Locus Coeruleus of Mood Disorder Patients Using a Multispectral Method to Prevent Interference with Immunocytochemical Signals by Neuromelanin.
10.1007/s12264-019-00339-y
- Author:
Lei GUO
1
;
Jochem STORMMESAND
2
;
Zheng FANG
1
;
Qingbin ZHU
1
;
Rawien BALESAR
2
;
Joop VAN HEERIKHUIZE
2
;
Arja SLUITER
2
;
Dick SWAAB
2
;
Ai-Min BAO
3
Author Information
1. Department of Neurobiology and Department of Neurology of the Second Affiliated Hospital, Institute of Neuroscience, NHC and CAMS Key Laboratory of Medical Neurobiology, Zhejiang University School of Medicine, Hangzhou, 310058, China.
2. Netherlands Institute for Neuroscience, Institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, 1105 BA, the Netherlands.
3. Department of Neurobiology and Department of Neurology of the Second Affiliated Hospital, Institute of Neuroscience, NHC and CAMS Key Laboratory of Medical Neurobiology, Zhejiang University School of Medicine, Hangzhou, 310058, China. baoaimin@zju.edu.cn.
- Publication Type:Journal Article
- Keywords:
Bipolar disorder;
ErbB4;
Locus coeruleus;
Major depressive disorder;
Tyrosine hydroxylase
- MeSH:
Aged;
Aged, 80 and over;
Bipolar Disorder;
metabolism;
pathology;
Depressive Disorder, Major;
metabolism;
pathology;
Female;
Humans;
Image Processing, Computer-Assisted;
Immunohistochemistry;
methods;
Locus Coeruleus;
metabolism;
pathology;
Male;
Melanins;
metabolism;
Microscopy;
methods;
Middle Aged;
Neurons;
metabolism;
pathology;
Receptor, ErbB-4;
metabolism;
Sensitivity and Specificity;
Spectrum Analysis;
methods;
Tyrosine 3-Monooxygenase;
metabolism
- From:
Neuroscience Bulletin
2019;35(2):205-215
- CountryChina
- Language:English
-
Abstract:
The locus coeruleus (LC) has been studied in major depressive disorder (MDD) and bipolar disorder (BD). A major problem of immunocytochemical studies in the human LC is interference with the staining of the immunocytochemical end-product by the omnipresent natural brown pigment neuromelanin. Here, we used a multispectral method to untangle the two colors: blue immunocytochemical staining and brown neuromelanin. We found significantly increased tyrosine hydroxylase (TH) in the LC of MDD patients-thus validating the method-but not in BD patients, and we did not find significant changes in the receptor tyrosine-protein kinase ErbB4 in the LC in MDD or BD patients. We observed clear co-localization of ErbB4, TH, and neuromelanin in the LC neurons. The different stress-related molecular changes in the LC may contribute to the different clinical symptoms in MDD and BD.
