Study on high throughput sequencing identification of Fructus Arctii and five counterfeit species mix power.
10.19540/j.cnki.cjcmm.20180703.012
- Author:
Yan-Ping XING
1
;
Si-You CHEN
1
;
Liang XU
1
;
Yong-Man LIANG
1
;
Jia-Hao WANG
1
;
Bing WANG
1
;
Tao LIU
2
;
Ting-Guo KANG
1
Author Information
1. Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
2. China Medical University, Shenyang 110122, China.
- Publication Type:Journal Article
- Keywords:
Fructus Arctii;
ITS2;
Illumina MiSeq;
mix power
- MeSH:
Arctium;
chemistry;
classification;
DNA Barcoding, Taxonomic;
DNA, Plant;
genetics;
DNA, Ribosomal Spacer;
genetics;
Drug Contamination;
Drugs, Chinese Herbal;
standards;
Fabaceae;
Fruit;
High-Throughput Nucleotide Sequencing;
Milk Thistle;
Onopordum;
Phylogeny;
Saussurea
- From:
China Journal of Chinese Materia Medica
2018;43(19):3862-3866
- CountryChina
- Language:Chinese
-
Abstract:
Fructus Arctii is a traditional Chinese medicine. The main counterfeit species are the seeds of Arctium tomentosum, Onopordum acanthium, Silybum marianum, Saussurea costus, Amorpha fruticosa. Traditional identification methods or molecular barcoding techniques can identify Fructus Arctii and its counterfeit species. However, the identification of the mixture of it and its spurious species is rarely reported. In this paper, we sequenced the ITS2 sequences of Fructus Arctii and 5 kinds of spurious species mix powder by high-throughput sequencing to identify the mixed powder species and providing new ideas for the identification of Fructus Arctii mix powder. The total DNA in mixed powder was extracted, and the ITS2 sequences in total DNA was amplified. Paired-end sequencing was performed on the DNA fragment of the community using the Illumina MiSeq platform. The sequence was analyzed by the software FLASH, QIIME and GraPhlAn etc. The results showed that the high quality ITS2 sequences of 39910 mix samples were obtained from the mixed samples, of which the total ITS2 sequence of the samples genus was 34 935. Phylogenetic analysis showed that the samples contained Fructus Arctii, A. tomentosum, O. acanthium, S. marianum, S. costus and A. fruticosa. Using ITS2 sequences as DNA barcodes, high-throughput sequencing technology can be used to detect the Fructus Arctii and its spurious specie in mixed powder, which can provide reference for the quality control, safe use of medicinal materials of Fructus Arctii and the identification of mixed powder of traditional Chinese medicine.
