Protective effect of Wuzi Yanzong recipe on testicular germ cell apoptosis in natural ageing rats through endoplasmic reticulum stress.
10.19540/j.cnki.cjcmm.20180530.001
- Author:
Na MA
1
;
Chang-Cheng ZHANG
1
;
Qian CHEN
1
;
Qiong-Yan MA
1
;
Xu YOU
1
;
Si-Qi YANG
1
;
Ding YUAN
2
;
Hai-Xia ZHAO
1
Author Information
1. Medical College of China Three Gorges University, Yichang 443002, China.
2. Ren-He Hospital of China Three Gorges University, Yichang 443002, China.
- Publication Type:Journal Article
- Keywords:
Wuzi Yanzong recipe;
ageing;
apoptosis;
endoplasmic reticulum stress;
testis
- MeSH:
Aging;
Animals;
Apoptosis;
drug effects;
Drugs, Chinese Herbal;
pharmacology;
Endoplasmic Reticulum Stress;
Germ Cells;
drug effects;
Male;
Rats;
Rats, Sprague-Dawley;
Testis;
drug effects
- From:
China Journal of Chinese Materia Medica
2018;43(19):3899-3904
- CountryChina
- Language:Chinese
-
Abstract:
To study the protective effects of Wuzi Yanzong recipe on testis germ cell apoptosis in natural ageing rats through endoplasmic reticulum stress (ERS), 16-month-old male SPF grade SD rats were randomly divided into three groups: ageing model group, and low and high-dose Wuzi Yanzong recipe groups (WZ, 1 and 4 g·kg⁻¹), with 10 rats in each group. In addition, 2-month-old SD male rats were used as adult control group. The ageing model group and the adult control group were fed with normal diet for 4 months. WZ groups were given the medicated feed for 4 months. After fasting for 12 hours, the rats were put to death. Then, the testes were immediately collected. The change of testicular tissue morphology was observed by HE staining. The expression levels of ER stress-related proteins GRP78, p-PERK, p-eif2, ATF4, p-IRE1, XBP1, ATF6 and apoptosis-related proteins CHOP, caspase12 and p-JNK in testes were detected by Western blot. Compared with the ageing model group, Wuzi Yanzong recipe alleviated the morphological changes of testicular tissue. Western blot results showed that Wuzi Yanzong recipe significantly increased the expression levels of endoplasmic reticulum stress-related proteins GRP78, p-PERK, p-eif2, ATF4, p-IRE1, XBP1, ATF6 and significantly decreased the expression levels of endoplasmic reticulum-induced apoptosis-related proteins CHOP, caspase 12 and p-JNK. In conclusion, Wuzi Yanzong recipe can alleviate the ageing-related apoptosis of testicular germ cells in natural ageing rats by regulating endoplasmic reticulum stress.
