Identification of metabolites of Danshensu in rats.
10.19540/j.cnki.cjcmm.20180709.010
- Author:
Xiao-Dan MEI
1
;
Yu-Qi WANG
1
;
Zi-Jian WANG
2
;
Wen-Jing ZHAO
1
;
Zhan-Peng SHANG
1
;
Yan-Jiang QIAO
2
;
Jia-Yu ZHANG
2
Author Information
1. School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China.
2. Beijing Institution of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
- Publication Type:Journal Article
- Keywords:
Danshensu;
UHPLC-LTQ-Orbitrap;
metabolic pathways;
metabolites
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Lactates;
blood;
metabolism;
urine;
Mass Spectrometry;
Rats;
Rats, Sprague-Dawley
- From:
China Journal of Chinese Materia Medica
2018;43(19):3933-3939
- CountryChina
- Language:Chinese
-
Abstract:
To identify the metabolites of Danshensu in plasma and urine in rats by using UHPLC-LTQ-Orbitrap method. After oral gavage of Danshensu CMC-Na suspension in SD rats, urine and plasma samples were collected and processed by solid phase extraction. ACQUITY UPLC BEH C₁₈ column (2.1 mm×100 mm, 1.7 μm) was utilized, with 0.1% formic acid (A)-acetonitrile (B) solution as the mobile phase for gradient elution. Negative electrospray ion mode based data-acquisition method was established to collect the mass spectrometry data of biological samples. As a result, Danshensu and 21 Danshensu Ⅰ phase and Ⅱ phase metabolites were finally identified according to the accurate mass measurements, mass fragmentation behaviors and comparing with the reference standards. The main metabolic pathways included dehydration, methylation, glucuronide conjugation, sulfate conjugation and their composite reactions. Consequently, our study expounded metabolites of Danshensu in rats based on UHPLC-LTQ-Orbitrap method and provided a reference for further researches on therapeutic material basis and mechanism of Danshensu.
