Fingerprinting and multi-indicator quantitative analysis of Mongolian drug Digeda-4 decoction.
10.19540/j.cnki.cjcmm.20180726.009
- Author:
Xiang TIAN
1
;
Rui-Xue DING
1
;
Gen-Na BA
2
;
Yu-Xia BAI
2
;
Na-Mu-Ji-la LAXI
2
;
Xiao-Yong RAO
1
;
Xiao-Jian LUO
1
Author Information
1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China.
2. Mongolian Medicine College, Inner Mongolia University for Nationalities, Tongliao 028000, China.
- Publication Type:Journal Article
- Keywords:
Mongolian medicine Digeda-4 decoction;
chemometrics methods;
fingerprint;
quantitative analysis
- MeSH:
Chromatography, High Pressure Liquid;
Cinnamates;
Drugs, Chinese Herbal;
analysis;
standards;
Iridoid Glucosides;
Iridoids;
Methanol;
Principal Component Analysis;
Quality Control
- From:
China Journal of Chinese Materia Medica
2018;43(19):3962-3969
- CountryChina
- Language:Chinese
-
Abstract:
To establish the high performance liquid chromatography (HPLC) fingerprint for Digeda-4 decoction (DGD-4D), determine the contents of aesculetin, geniposide, picroside Ⅰ, picroside Ⅱ and ellagicacid in DGD-4D, and provide the scientific foundation for quality control of DGD-4D. The analysis was performed on Diamonsil(2) C₁₈ (4.6 mm×250 mm,5 μm) column, with methanol-0.1% phosphoric acid aqueous solution as mobile phase for gradient elution. The flow rate was 1.0 mL·min⁻¹; injection size was 10 μL; temperature was maintained at 30 °C, and the detection wavelength was set at 254 nm. The common mode of DGD-4D HPLC fingerprint was established, and the hidden information was analyzed by Chemometrics. Chromatographic peaks for DGD-4D were identified by HPLC and quantitative analysis was conducted for characteristic peaks. There were 17 common peaks in the fingerprints and the similarity of the fingerprints was over 0.9 in all 15 batches. The samples were broadly divided into four kinds by principal component analysis and clustering analysis. Four marker compounds were verified by partial least squares discriminant analysis, and No. 9, 12 and 14 peaks were identified as geniposide, picroside Ⅱ, and picroside Ⅰ respectively. The average recoveries were in the range of 95.91%-97.31%. The HPLC fingerprint method for content determination is reliable, accurate, rapid, simple, and reproducible, and can be used as one of the effective methods to control the quality of DGD-4D.
