Establishment of a quantitative real-time PCR for rapid detection of Panax quinquefolius.

Author: Yong-Hui HUANG ¹ ; Miao-Feng XU ¹ ; Yong-Bing QUAN ¹ ; Yuan-Li CHI ¹ ; Wei LIN ¹ ; Li LIAO ¹ ; Wei-Dong ZHANG ¹
Author Information

1. Technology Center of Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai 519000, China.
Publication Type:Journal Article
Keywords: Panax ginseng; Panax quinquefolius; quantitative real-time PCR; rapid identification
MeSH: DNA Primers; DNA Probes; Drugs, Chinese Herbal; standards; Panax; genetics; Real-Time Polymerase Chain Reaction
From: China Journal of Chinese Materia Medica 2018;43(20):4050-4054
CountryChina
Language:Chinese
Abstract: In this study, the specific primers and probes of Panax quinquefolius were designed for a quantitative real-time PCR, and the rapid identification method of P. quinquefolius was established by optimizing conditions. The method was used to validate 43 samples of the traditional Chinese medicine,and the results showed that 22 samples of P. quinquefolius were identified accurately. The limit of detection of the method can be reach to 1×10⁻⁴ ng. The method is accurate, fast, sensitive and specifically.