Comparative analysis of multiple index constituents in Ophiopogonis Radix and Liriopes Radix.
10.19540/j.cnki.cjcmm.20180702.009
- Author:
Meng-Xia TAN
1
;
Jia-Li CHEN
1
;
Li-Si ZOU
1
;
Xun-Hong LIU
1
;
Ren-Mao TANG
2
;
Ji-Mei MA
2
;
Shu-Yu CHEN
1
;
Jing-Jing SHI
1
Author Information
1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
2. SZYY Group Pharmaceutical Limited, Taizhou 225500, China.
- Publication Type:Journal Article
- Keywords:
Liriopes Radix;
Ophiopogonis Radix;
UFLC-QTRAP-MS/MS;
content determination;
multivariate statistical analysis
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
analysis;
Liriope Plant;
chemistry;
Ophiopogon;
chemistry;
Phytochemicals;
analysis;
Plant Roots;
chemistry;
Saponins;
analysis;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2018;43(20):4084-4092
- CountryChina
- Language:Chinese
-
Abstract:
An analytical method based on UFLC-QTRAP-MS/MS was established for simultaneous determination of thirty-three components including steroidal saponins, homoisoflavonoids, amino acids and nucleosides in Ophiopogonis Radix. Thirty-three target components of commercial medicinal materials of Maidong were comparative analysis. Synergi™ Hydro-RP 100 column (2.0 mm × 100 mm, 2.5 μm) was used with 0.1% formic acid solution-0.1% formic acid acetonitrile for gradient elution at a flow rate of 0.4 mL·min⁻¹. In addition, multiple reaction monitoring (MRM) mode was employed. The data were comprehensively processed and analyzed with hierarchical clustering analysis(HCA), principal component analysis(PCA) and partial least squares discriminant analysis(PLS-DA) methods. All components showed good linearity(>0.999 0) within the tested ranges. The average recoveries were between 96.23%-102.0%, and the relative standard deviation(RSD) were less than 5%. The results showed that there were significant differences in components between Ophiopogonis Radix and Liriopes Radix, with seven components obviously different. This method was useful for providing basis for the comprehensive evaluation and intrinsic quality control of Ophiopogonis Radix and Liriopes Radix , and may provide a new method reference for the identification of Ophiopogonis Radix and Liriopes Radix.
