Transport characteristics of Shuxiong prescription across Caco-2 cell monolayer model.
10.19540/j.cnki.cjcmm.20180709.004
- Author:
Bin-Bin FENG
1
;
Jian-Hai ZHANG
1
;
Fei LI
2
Author Information
1. Chongqing Three Gorges Medical College, Chongqing 404120, China.
2. Third Military Medical University, Chongqing 400038, China.
- Publication Type:Journal Article
- Keywords:
Caco-2;
P-gp glycoprotein;
Shuxiong prescription;
transport
- MeSH:
Biological Transport;
Caco-2 Cells;
Chromatography, High Pressure Liquid;
Digoxin;
pharmacokinetics;
Drugs, Chinese Herbal;
pharmacokinetics;
Humans
- From:
China Journal of Chinese Materia Medica
2018;43(20):4132-4137
- CountryChina
- Language:Chinese
-
Abstract:
Shuxiong prescription (Notoginseng Radix et Rhizoma, Chuanxiong Rhizome and Carthami Flos) has the function of activating blood circulation to dissipate blood stasis, activating meridians to stop pain. This paper was mainly aimed to discuss the transport characteristics of Shuxiong prescription across Caco-2 cell monolayer. Safe concentration range of Shuxiong prescription against Caco-2 cell monolayer model was determined by MTT assay. The mechanism of Shuxiong prescription bidirectional transport was investigated by Caco-2 cell monolayer model. The apparent permeability coefficient Papp of digoxin was determined by high performance liquid chromatography (HPLC). The test results showed that the Papp of extract from Notoginseng Radix et Rhizoma, Chuanxiong Rhizome, Carthami Flos, Chuanxiong Rhizome+Carthami Flos and Shuxiong prescription transport from apical (AP) side to basolateral (BL) side was (3.12±0.73)×10⁻⁶, (2.58±0.41)×10⁻⁶, (4.97±0.64)×10⁻⁶, (4.63±0.57)×10⁻⁶, (5.79±0.68)×10⁻⁶ cm·s⁻¹, respectively, indicating that the transport of digoxin across Caco-2 cell monolayer model was active absorption, and the P-gp protein took part in the process. Chuanxiong Rhizome could significantly decrease the transport of digoxin from BL→AP(<0.01) and increase its transport from AP→BL(<0.05) significantiy. After the addition of Shuxiong prescription, the transport of digoxin from BL→AP was significantly inhibited(<0.01). The results suggested that the extract of safflower had no effect on P-gp transport, nor on the independence diffusion of digoxin. The transport of digoxin could be degraded by the extract of Chuanxiong Rhizome and the extract of Shuxiong prescription from BL→AP(<0.01), significantly; pseudo-ginseng had no effect on the independence diffusion of digoxin; the extract of safflower+Chuanxiong Rhizome had the same experimental result as Chuanxiong Rhizome extract.
