Rapid analysis on chemical constituents in Qige Keli by RRLC-Q-TOF-MS/MS.
10.19540/j.cnki.cjcmm.20180807.003
- Author:
Hui-Min YIN
1
;
Wen-Zhe HUANG
2
;
Wei XIAO
1
Author Information
1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
2. Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China.
- Publication Type:Journal Article
- Keywords:
Qige Keli;
RRLC-Q-TOF-MS/MS;
flavonoids;
lactones;
organic acids;
saponins
- MeSH:
Acids;
analysis;
Drugs, Chinese Herbal;
chemistry;
Flavonoids;
analysis;
Glycosides;
analysis;
Lactones;
analysis;
Phytochemicals;
analysis;
Saponins;
analysis;
Spectrometry, Mass, Electrospray Ionization;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2018;43(21):4240-4247
- CountryChina
- Language:Chinese
-
Abstract:
The qualitative analysis method of RRLC-Q-TOF-MS/MS was established for determine the chemical constituents in Qige Keli. Kramosil C₁₈ column (4.6 mm×150 mm, 3.5 μm) was used at the temperature of 30 °C. The mobile phase was 0.2% formic acid and acetonitrile by gradient elution, with a flow rate at 1.0 mL·min⁻¹, and the injection volume was 10 μL. The high-resolution quadrupole time-flight mass spectrometry was used as detector with electrospray ion source in both positive and negative models. On the basis of medicinal materials, reference materials, literature reports, and mass spectrometry data, the chemical composition in the Qige Keli was identified. A total of 44 compounds were identified, including 3 flavonoids, 21 flavonoid glycosides, 8 organic acids, 6 lactones, and 3 saponins. The results laid the foundation for the quality control of Qige Keli and the further research on pharmacodynamic materials.
