Phenolic constituents from stems of Ilex asprella.
10.19540/j.cnki.cjcmm.20180726.006
- Author:
Jian-Yong XING
1
;
Bing-Zhao DU
1
;
Xiao FENG
1
;
He-Xin-Ge ZHANG
1
;
Zheng-Zhou HAN
2
;
Zeng-Ping GAO
1
;
Peng-Fei TU
1
;
Xing-Yun CHAI
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
2. China Resources Sanjiu Medical & Pharmaceutical Co., Ltd., Shenzhen 518029, Chin.
- Publication Type:Journal Article
- Keywords:
Aquifoliaceae;
Ilex;
Ilex asprella;
anti-inflammatory activity;
phenolic acids
- MeSH:
Animals;
Anti-Inflammatory Agents;
isolation & purification;
pharmacology;
Ilex;
chemistry;
Mice;
Nitric Oxide;
metabolism;
Phenols;
chemistry;
Phytochemicals;
isolation & purification;
pharmacology;
Plant Stems;
chemistry;
RAW 264.7 Cells
- From:
China Journal of Chinese Materia Medica
2018;43(21):4267-4273
- CountryChina
- Language:Chinese
-
Abstract:
Phytochemical investigation on Ilex asprella stems by using various chromatographic techniques led to the isolation of 18 phenolic constituents. Based on spectroscopic data analyses and/or comparison of the spectroscopic data with those in literature, these constituents were identified, including two lignans (1, 2), five phenylpropanes (3-7), six chlorogenic analogues (8-13), and five benzoic analogues (14-18). Among them, compounds 3-7, 9, 11, 13, 14, 17, and 18 were isolated from genus Ilex for the first time, and 2, 8, 10, 15, and 16 were isolated from this species for the first time. The in vitro anti-inflammatory assay results showed that compounds 8, 9, 11, 13, and 15 possessed moderate inhibition on the NO production in RAW264.7 cells with IC₅₀ values of 51.1-85.8 μmol·L⁻¹. The present study brought preliminary reference for the clarification of therapeutic ingredients of I. asprella with anti-inflammatory efficacy and its quality evaluation.
