Determination of three anthroic acids in petri dish cultured Antrodia camphorate by quantitative analysis of multi-component with single marker.
10.19540/j.cnki.cjcmm.2018.0114
- Author:
Yu WU
1
;
Wen-Jing TIAN
1
;
Pei-Xin LIN
2
;
Ji-Meng LUO
2
;
Ting LIN
1
;
Hai-Feng CHEN
1
Author Information
1. School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China.
2. Industrial Technology Research Institute of Taiwan, Xinzhu 30011, China.
- Publication Type:Journal Article
- Keywords:
(25S)-antcin C;
(25S)-antcin K;
multi-component with single marker;
petri dish cultured Antrodia camphorate;
relative correction factor(RCF)
- MeSH:
Antrodia;
chemistry;
Biomarkers;
analysis;
Cholestenes;
analysis;
Chromatography, High Pressure Liquid
- From:
China Journal of Chinese Materia Medica
2018;43(21):4283-4287
- CountryChina
- Language:Chinese
-
Abstract:
The present study is establish the quantitative analysis of multi-component with single marker for determining three anthroic acids, (25S)-antcin K, (25R)-antcin K and (25S)-antcin C in the petri dish cultured Antrodia camphorata. The relative correction factors of (25S)-antcin K and (25R)-antcin K were established by high performance liquid chromatography with (25S)-antcin C as the internal reference. Relative correction factors were used to calculate the contents of (25S)-antcin K and (25R)-antcin K which were difficult to gain in abundance. At the same time, the contents of these three compounds were determined by external standard method. Two methods were compared to evaluate the accuracy and rationality of the multi-components with single marker method in the determination of the petri dish cultured A. camphorate. It was found that the quantitative method of multi-component with single marker and external standard method showed no significant difference. In summary, taking (25S)-antcin C as the internal reference, the method of multi-component with single marker can be applied to the quantitative analysis of (25S)-antcin K and (25R)-antcin K in the petri dish cultured A. camphorata.
