Rapid identification of chemical components in Xingbei Zhike Keli by UPLC-Q-TOF-MS/MS.
10.19540/j.cnki.cjcmm.20180807.004
- Author:
Ning ZHANG
1
;
Xia GAO
2
;
Yu ZHOU
2
;
Ting GENG
2
;
Biao YANG
2
;
Xue WANG
2
;
Wen-Zhe HUANG
2
;
Wei XIAO
1
Author Information
1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
2. Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China.
- Publication Type:Journal Article
- Keywords:
UPLC-Q-TOF-MS/MS;
Xingbei Zhike Keli;
chemical composition spectrum
- MeSH:
Alkaloids;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Flavonoids;
Spectrometry, Mass, Electrospray Ionization;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2018;43(22):4439-4449
- CountryChina
- Language:Chinese
-
Abstract:
To analyze and identify the chemical components in Xingbei Zhike Keli by using ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF-MS/MS). The analysis was performed on Agilent Zorbax SB-C₁₈(4.6 mm×250 mm, 5 μm) column, with methanol-0.08% formic acid solution (including 0.1% ammonium formate) as the mobile phase for gradient elution. The flow rate was 1 mL·min⁻¹ and column temperature was 30 °C. The MS spectrum was acquired in both negative and positive ion modes by using electron spray ionization (ESI). These components were further analyzed based on accurate m/z, secondary fragmentation and other information combined with reference substance and literature data. As a result, 87 compounds were successfully identified and predicted, including alkaloids, flavonoids, coumarins and saponins, of which 23 compounds were verified by comparing with reference substances. These results provide reference for the quality control of Xingbei Zhike Keli, and lay the foundation for elucidating their effective components and mechanism of action.
