Analysis of flavonoids and antitumor activity of transgenic Saussurea involucrate.
10.19540/j.cnki.cjcmm.20180619.001
- Author:
Zhi-Xiu CAO
1
;
Hou-Hua LI
1
;
Ai LI
1
;
Peng-Yuan LIU
1
;
Yan-Ying ZHAO
2
;
Pei-Qi MAO
1
;
Gui-Lin LI
1
Author Information
1. College of Landscape Architecture and Arts, Northwest A & F University, Yangling 712100, China.
2. Nanjing International Medical Technology Services Limited, Nanjing 210000, China.
- Publication Type:Journal Article
- Keywords:
Saussurea involucrata;
anthocyanins;
antitumor activity;
apoptosis
- MeSH:
Apoptosis;
Chromatography, High Pressure Liquid;
Flavonoids;
Humans;
Phenols;
Plant Extracts;
Saussurea
- From:
China Journal of Chinese Materia Medica
2018;43(22):4491-4497
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to investigate the flavonoids of callus of transgenic and non-transgenic Saussurea involucrate and its antitumor activity on the esophageal cancer CaEs-17 cells. The species and content of mono-phenols were detected by high performance liquid chromatography. The growth of human esophageal cancer CaEs-17 cells was detected using CCK-8 assay, apoptosis morphology observation and flow cytometry. Expression of related apoptotic genes Bax and Bcl-2 were determined by qPCR. The results showed that the content of total flavonoids in the transgenic callus was 2.72 times that of the non-transgenic callus. The cyanidin-galactoside was detected in the transgenic callus, but not in the non-transgenic callus. The inhibitory effect of the extracts from the transgenic callus on CaEs-17 cells was more significant than that of the non-transgenic callus, and the IC₅₀ value had a decreased of 26.4%. Flow cytometry analysis results showed that the apoptosis induction effect of the extracts from the transgenic callus on CaEs-17 cells was significantly better than that of the non-transgenic callus. Fluorescence quantitative PCR analysis results showed that the extracts from the transgenic callus could up-regulate the expression of proapoptotic gene Bax and down-regulate the expression of apoptotic gene Bcl-2, and the regulation effect of the transgenic callus was more significant. Therefore, compared with the non-transgenic callus, the antitumor activity of the extracts from the callus of transgenic S. involucrate on the esophageal cancer CaEs-17 cells was significantly increased, which was closely related to the accumulation of cyanidin-galactoside and its metabolism-related flavonoid compounds in the transgenic callus.
