Effects of Honokiol on cognitive function in mice with kainic acid-induced epilepsy.
- Author:
Qingmei WANG
1
;
Min SHU
1
;
Qianzi XU
1
;
Yiyi XIE
1
;
Shengzhe RUAN
1
;
Jianda WANG
2
;
Linghui ZENG
1
Author Information
1. School of Medicine, Zhejiang University City College, Hangzhou 310015, China.
2. Department of Pediatrics, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Biphenyl Compounds;
pharmacology;
Cognition;
drug effects;
Epilepsy;
chemically induced;
Gene Expression Regulation;
drug effects;
Hippocampus;
drug effects;
Kainic Acid;
Lignans;
pharmacology;
Male;
Malondialdehyde;
Maze Learning;
drug effects;
Mice;
Mice, Inbred ICR;
Neurons;
drug effects;
Superoxide Dismutase;
genetics
- From:
Journal of Zhejiang University. Medical sciences
2018;47(5):450-456
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of Honokiol on cognitive function in mice with epilepsy.
METHODS:Kainic acid (38 mg/kg) was intraperitoneally injected in 5 weeks old male ICR mice to induce epilepsy. Honokiol at dose of 3, 10, 30 mg/kg was given to epilepic mice by intraperitoneal injection for 10 days. Fluoro-Jade B staining was used to assess neuronal death; Morris water maze and Y maze tests were used to measure cognitive function such as learning and memory; Western blot was performed to detect the expression of acetylated superoxide dismutase (SOD), microtubule associated protein 1 light chain 3-Ⅱ (LC3-Ⅱ) and P62 in hippocampus tissue; thiobarbituric acid and WST-1 methods were used to detect malondialdehyde (MDA) and SOD.
RESULTS:Compared with control group, the levels of acetylated-SOD, MDA, LC3-Ⅱ, P62 and neuronal death increased, cognitive function and SOD decreased in model group (<0.05 or <0.01). Honokiol at the dose of 10 mg/kg and 30 mg/kg decreased SOD acetylation, MDA content, expression of LC3-Ⅱ and P62, as well as neuronal death, and the cognitive function was improved (<0.05 or <0.01), especially in 30 mg/kg Honokiol group.
CONCLUSIONS:Honokiol alleviates oxidative stress and autophagy degradation disorder, decreases neuronal death, and therefore improves cognitive function in epilepsy mice.
