Protective effect of curcumin on dopamine neurons in Parkinson's disease and its mechanism.
- Author:
You WU
1
;
Shunli LIANG
1
;
Bin XU
1
;
Rongbo ZHANG
1
;
Linsheng XU
1
Author Information
1. Department of Neurology, the Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310005, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line;
Curcumin;
pharmacology;
Dopaminergic Neurons;
drug effects;
Humans;
Mice;
Mice, Inbred C57BL;
Parkinson Disease;
alpha-Synuclein;
metabolism
- From:
Journal of Zhejiang University. Medical sciences
2018;47(5):480-486
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of curcumin on dopamine neurons in Parkinson's disease (PD) and its mechanism.
METHODS:SH-SY5Y human neuroblastoma cells were treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to establish the PD cell model. The model cells were treated with curcumin and/or autophagy inhibitor 3-MA. After 48 h of drug treatment, the number of surviving dopamine neurons was detected by tyrosine hydroxylase immunofluorescence method. Western blotting was used to detect protein expression of α-Synuclein (α-Syn), transcription factor EB (TFEB) and autophagy-related proteins lysosome-associated membrane protein 2A (LAMP2A) and microtubule-associated protein 1 light chain 3-Ⅱ(LC3-Ⅱ); RT-PCR was used to detect mRNA expression of α-Syn.
RESULTS:Compared with MPTP model group, curcumin increased the number of surviving dopamine neurons(<0.01), decreased both protein expression and mRNA expression of α-Syn (all <0.01), and increased protein expression of TFEB, LAMP2A and LC3-Ⅱ (all <0.01). When curcumin and 3-MA were given concurrently, the number of surviving dopamine neurons, protein expression of TFEB, LAMP2A and LC3-Ⅱ increased (<0.05 or <0.01), and both protein expression and mRNA expression of α-Syn decreased (<0.05 or <0.01) compared with MPTP model group; but the number of surviving dopamine neurons and protein expression of LAMP2A and LC3-Ⅱ decreased compared with curcumin group (all <0.05).
CONCLUSIONS:Curcumin exerts protective effect on dopamine neurons in PD, which may be associated with enhancing autophagy and promoting the clearance of α-Syn.
