Establishment of cell lines for quality control of prenatal genetic diagnosis by gene transfection.
- Author:
Binghuan WENG
1
;
Wei XU
2
;
Lan SU
1
;
Min SHEN
1
;
Rong LI
3
;
Xiaopeng XU
2
;
Lanjuan LI
2
Author Information
1. Key Laboratory of Reproductive Genetics of the Ministry of Education, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
2. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
3. Shulan(Hangzhou) Hospital, Hangzhou 310004, China.
- Publication Type:Journal Article
- MeSH:
Antigens, Polyomavirus Transforming;
genetics;
Cell Line;
Female;
Genetic Vectors;
Humans;
Karyotype;
Pregnancy;
Prenatal Diagnosis;
methods;
Quality Control;
Recombinant Proteins;
genetics;
Transfection
- From:
Journal of Zhejiang University. Medical sciences
2018;47(5):520-524
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a cell lines for quality control of prenatal genetic diagnosis.
METHODS:The recombined SV40LTag-pcDNA3.1(-) vector was constructed and transfected by lipidosome into human amniotic fluid cells with common aneuploidy. Positive clones were screened by G418, and the immortality of transfected cell line was identified.
RESULTS:Cell line with karyotype of 46, XY, t(8;19)(q24.3;q13.1) from primary amniotic fluid cells was established. Karyotype analytical results indicated that the cell line at its 15th generation maintained the same karyotype of its primary cell.
CONCLUSIONS:Gene can lead to immortality of amniotic fluid cells, which contributes to preparing cell lines for internal and external quality control in prenatal genetic diagnosis.
