Metabonomics studies of urine from mice with early-stage Alzheimer's disease.
- Author:
Yongquan ZHENG
1
;
Xiaoqian ZHANG
2
;
Jiuxia CHEN
3
;
Qi ZHOU
3
;
Hongchang GAO
3
Author Information
1. Department of Pharmacy, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
2. Department of Pharmacy, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
3. School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
- Publication Type:Journal Article
- MeSH:
Alzheimer Disease;
urine;
Animals;
Disease Models, Animal;
Magnetic Resonance Spectroscopy;
Metabolome;
Metabolomics;
Mice;
Mice, Inbred C57BL
- From:
Journal of Zhejiang University. Medical sciences
2018;47(6):636-642
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the metabolic profiles of urine from mice with early-stage Alzheimer's disease (AD).
METHODS:Urine samples were collected from 13 mice of 16 weeks and 15 wild-type mice. H-NMR spectroscopy was acquired with a one-dimensional NOESY pulse sequence, and the integral values were imported to SIMCA-P+12.0 software for analysis.
RESULTS:The metabonomic analysis showed that the metabolic profiles of the mice were significantly different from that of age-matched wild-type mice. The levels of 3-hydroxybutyrate, 2-hydroxybutyrate, succinic acid, 2-ketoglutaric acid, citric acid, cis-aconitic acid, fumaric acid decreased, and those of acetic acid, trimethylamine, taurine, creatinine, hippuric acid, formic acid, trigonelline, urea increased (all <0.05).
CONCLUSIONS:Metabolic pathways including glucose metabolism and methylamine metabolism may be involved in the pathogenesis of early AD.
