Single-particle cryo-electron microscopy opens new avenues in structural biology of G protein-coupled receptor.
- Author:
Chuntao LI
1
;
Huibing ZHANG
1
;
Yan ZHANG
1
Author Information
1. Department of Biophysics, Zhejiang University School of Medicine, Hangzhou 310058, China.
- Publication Type:Journal Article
- MeSH:
Cryoelectron Microscopy;
Protein Binding;
Protein Structure, Tertiary;
Receptors, G-Protein-Coupled;
chemistry
- From:
Journal of Zhejiang University. Medical sciences
2019;48(1):39-43
- CountryChina
- Language:Chinese
-
Abstract:
G protein-coupled receptors(GPCRs)represent the largest class of cell surface receptors,mediating wide range of cellular and physiological processes through their transducers,G proteins and the-arrestins participate in almost all pathological processes. Recent technological advances are revolutionizing the utility of cryo-electron microscopy(cryo-EM),leading to a tremendous progress in the structural studies of biological macromolecules and cryo-EM has played a leading role in the structural biology of GPCR signaling complex. New discoveries of high-resolution threedimensional structures of GPCR signaling complexes based on cryo-EM have emerged vigorously,which depict the common structural characteristics of intermolecular interaction between GPCR and G protein complex-the conformational changes of the transmembrane helix 6 of receptors,and also demonstrate the structural basis of G protein subtype selectivity. Single-particle cryo-EM becomes an efficient tool for identifying the molecular mechanism of receptor-ligand interaction,providing important information for understanding GPCR signaling and the structure-based drug design.
