High dose vitamin C inhibits proliferation of breast cancer cells through reducing glycolysis and protein synthesis.
- Author:
Qingmei WANG
1
;
Qianzi XU
1
;
Anyi WEI
1
;
Shishuo CHEN
1
;
Chong ZHANG
1
;
Linghui ZENG
1
Author Information
1. School of Medicine, Zhejiang University City College, Hangzhou 310015, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Ascorbic Acid;
pharmacology;
Breast Neoplasms;
drug therapy;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Glycolysis;
drug effects;
Humans;
Mice;
Mice, Nude;
Protein Biosynthesis;
drug effects
- From:
Journal of Zhejiang University. Medical sciences
2019;48(3):296-302
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of high dose vitamin C (VC) on proliferation of breast cancer cells and to explore its mechanisms.
METHODS:Human breast cancer cells Bcap37 and MDA-MB-453 were treated with VC at low dose (0.01 mmol/L), medium dose (0.10 mmol/L) and high dose (2.00 mmol/L). Cell proliferation was determined with CCK-8 assay, protein expression was evaluated by Western blot, and the secretion of lactic acid in tumor cells was detected by colorimetric method. Bcap37 cells were inoculated in nude mice, and tumor baring nude mice were intraperitoneally injected with high VC(4 g/kg, VC group, =5)or normal saline (control group, =5) for 24 d. Tumor weight and body weight were calculated.
RESULTS: experiments demonstrated that high dose VC significantly inhibited cell proliferation in Bcap37 and MDA-MB-453 cells (all <0.01); the expressions of Glut1 and mTOR signaling pathway-related proteins were decreased (all <0.05); and the secretion of lactic acid was also markedly reduced (all <0.05). experiment showed that the tumor weight was decreased in mice treated with high-dose VC as compared with control group (<0.05), but no difference in body weights between two groups was observed.
CONCLUSIONS:High dose VC may inhibit proliferation of breast cancer cells both and through reducing glycolysis and protein synthesis.
