Correlation of phosphorylated S6 protein expression in blood and brain tissue in mice and rats with kainic acid-induced seizure.
- Author:
Miao FAN
1
;
Shuminin DONG
1
;
Xinyi ZOU
1
;
Boyuan ZHENG
1
;
Yurun HUANG
1
;
Jianda WANG
2
;
Linghui ZENG
1
Author Information
1. School of Medicine, Zhejiang University City College, Hangzhou 310015, China.
2. Department of Paediatrics, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Brain;
drug effects;
physiopathology;
Gene Expression Regulation;
drug effects;
Kainic Acid;
Mice;
Mice, Inbred C57BL;
Phosphorylation;
Rats;
Rats, Sprague-Dawley;
Seizures;
blood;
chemically induced;
physiopathology
- From:
Journal of Zhejiang University. Medical sciences
2019;48(3):303-309
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the correlation of phosphorylated ribosomal S6 protein (P-S6) content in blood and brain tissue in mice and rats with seizure.
METHODS:Seizure models were induced by intraperitoric injection of kainic acid (KA) in C57BL/mice and SD rats. Flow cytometry was used to detect the content of P-S6 in blood; Western blot was used to detect the expression of P-S6 in brain tissues. The correlation between P-S6 expression in blood and in brain tissue was examine by Pearson analysis, and the correlation between P-S6 expression in blood and the severity of seizure was also observed.
RESULTS:Western blotting analysis showed that the expression of P-S6 was significantly increased in peripheral blood and brain tissue in mice 1 h after KA-induced seizure,and the expression levels increased to (1.49±0.45) times (<0.05) and (2.55±0.66) times ( <0.01) of the control group, respectively. Flow cytometry showed that the positive percentage and average fluorescence intensity of P-S6 in the blood of mice increased significantly 1 h after KA-induced seizures (<0.01), which was consistent with the expression of P-S6 in brain tissue (=0.8474, <0.01). Flow cytometry showed that the average fluorescence intensity of P-S6 in blood increased from 14.89±9.75 to 52.35±21.72 (<0.01) in rats with seizure, which was consistent with the change of P-S6 in brain tissue (=0.9385, <0.01). Rats with higher levels of seizure were of higher levels of P-S6 in peripheral blood.
CONCLUSIONS:Consistent correlation of P-S6 expression is demonstrated in peripheral blood and in brain tissue after KA-induced seizure, suggesting that the expression of P-S6 in blood can accurately reflect the changes of mTOR signaling pathway in brain tissue.
