The effect of noise exposure on insulin sensitivity in mice may be mediated by the JNK/IRS1 pathway.
10.1186/s12199-018-0694-3
- Author:
Lijie LIU
1
;
Cong FANG
2
;
Jing YANG
2
;
Hongyu ZHANG
2
;
Yi HUANG
2
;
Chuanying XUAN
2
;
Yongfang WANG
3
;
Shengwei LI
3
;
Jun SHA
3
;
Mingming ZHA
3
;
Min GUO
3
Author Information
1. Medical College, Southeast University, No.87, Dingjiaqiao Street, Gulou, Nanjing, China. liulijie1973@163.com.
2. Institute of Life Sciences, Southeast University, Nanjing, China.
3. Medical College, Southeast University, No.87, Dingjiaqiao Street, Gulou, Nanjing, China.
- Publication Type:Journal Article
- Keywords:
Inflammation;
Insulin sensitivity;
JNK/IRS1 pathway;
Noise exposure;
Oxidative stress
- MeSH:
Animals;
Biomarkers;
metabolism;
Inflammation;
physiopathology;
Insulin Receptor Substrate Proteins;
genetics;
metabolism;
Insulin Resistance;
genetics;
immunology;
MAP Kinase Signaling System;
physiology;
Male;
Mice;
Mice, Inbred ICR;
Mitogen-Activated Protein Kinase 8;
genetics;
metabolism;
Noise;
adverse effects;
Oxidative Stress;
physiology;
Proto-Oncogene Proteins c-akt;
genetics;
metabolism;
Random Allocation;
Time Factors
- From:Environmental Health and Preventive Medicine
2018;23(1):6-6
- CountryJapan
- Language:English
-
Abstract:
BACKGROUND:Epidemiological studies have suggested that noise exposure may increase the risk of type 2 diabetes mellitus (T2DM), and experimental studies have demonstrated that noise exposure can induce insulin resistance in rodents. The aim of the present study was to explore noise-induced processes underlying impaired insulin sensitivity in mice.
METHODS:Male ICR mice were randomly divided into four groups: a control group without noise exposure and three noise groups exposed to white noise at a 95-dB sound pressure level for 4 h/day for 1, 10, or 20 days (N1D, N10D, and N20D, respectively). Systemic insulin sensitivity was evaluated at 1 day, 1 week, and 1 month post-noise exposure (1DPN, 1WPN, and 1MPN) via insulin tolerance tests (ITTs). Several insulin-related processes, including the phosphorylation of Akt, IRS1, and JNK in the animals' skeletal muscles, were examined using standard immunoblots. Biomarkers of inflammation (circulating levels of TNF-α and IL-6) and oxidative stress (SOD and CAT activities and MDA levels in skeletal muscles) were measured via chemical analyses.
RESULTS:The data obtained in this study showed the following: (1) The impairment of systemic insulin sensitivity was transient in the N1D group but prolonged in the N10D and N20D groups. (2) Noise exposure led to enhanced JNK phosphorylation and IRS1 serine phosphorylation as well as reduced Akt phosphorylation in skeletal muscles in response to exogenous insulin stimulation. (3) Plasma levels of TNF-α and IL-6, CAT activity, and MDA concentrations in skeletal muscles were elevated after 20 days of noise exposure.
CONCLUSIONS:Impaired insulin sensitivity in noise-exposed mice might be mediated by an enhancement of the JNK/IRS1 pathway. Inflammation and oxidative stress might contribute to insulin resistance after chronic noise exposure.
