Red blood cell acetylcholinesterase activity among healthy dwellers of an agrarian region in Sri Lanka: a descriptive cross-sectional study.
10.1186/s12199-018-0717-0
- Author:
Devarajan RATHISH
1
;
Indika SENAVIRATHNA
2
;
Channa JAYASUMANA
3
;
Suneth AGAMPODI
4
Author Information
1. Department of Pharmacology, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Saliyapura, Sri Lanka. rathishdeva@gmail.com.
2. Department of Biochemistry, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Saliyapura, Sri Lanka.
3. Department of Pharmacology, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Saliyapura, Sri Lanka.
4. Department of Community Medicine, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Saliyapura, Sri Lanka.
- Publication Type:Journal Article
- Keywords:
Acetylcholinesterase activity;
Carbamate;
Healthy volunteers;
Organophosphate
- MeSH:
Acetylcholinesterase;
blood;
Adult;
Agriculture;
Cross-Sectional Studies;
Erythrocytes;
enzymology;
Female;
Humans;
Male;
Middle Aged;
Sri Lanka;
Young Adult
- From:Environmental Health and Preventive Medicine
2018;23(1):25-25
- CountryJapan
- Language:English
-
Abstract:
BACKGROUND:Assessment of acetylcholinesterase-inhibitor insecticide (AChEII) toxicity depends on the measurement of red blood cell acetylcholinesterase (RBC-AChE) activity. Its interpretation requires baseline values which is lacking in scientific literature. We aim to find the measures of central tendency and variation for RBC-AChE activity among dwellers of Anuradhapura, where the use and abuse of AChEIIs were rampant for the last few decades.
METHODS:A descriptive cross-sectional study with a community-based sampling for 100 healthy non-farmers (male:female = 1:1) was done using pre-determined selection criteria. Duplicate measurements of RBC-AChE activity were performed according to the modified Ellman procedure. Pearson's correlation and regression analysis were sort for RBC-AChE activity against its possible determinants.
RESULTS:RBC-AChE activity had a mean of 449.8 (SD 74.2) mU/μM Hb with a statistical power of 0.847. It was similar to values of "healthy controls" from previous Sri Lankan toxicological studies but was low against international reference value [586.1 (SD 65.1) mU/μM Hb]. None of the possible determinants showed a significant strength of relationship with RBC-AChE activity.
CONCLUSION:The baseline RBC-AChE activity among people of Anuradhapura is low in comparison with international reference values. This arises a need to find a causative mechanism.
