Effect and molecular mechanism of interferon-α on podocyte apoptosis induced by hepatitis B virus X protein.
- Author:
Yong-Hong SUN
1
;
Xiao-Yan LEI
;
Xing-Xing CHEN
;
Wei-Jing CUI
;
Jing LIU
Author Information
1. Department of Pediatrics, Gansu Province People's Hospital, Lanzhou 730000, China. leixiaoyan601@163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Hepatitis B virus;
Interferon-alpha;
Mice;
Podocytes;
Trans-Activators
- From:
Chinese Journal of Contemporary Pediatrics
2019;21(9):930-935
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect and molecular mechanism of interferon-α (INF-α) on the apoptosis of the mouse podocyte cell line MPC5 induced by hepatitis B virus X (HBx) protein.
METHODS:MPC5 cells were transfected with the pEX plasmid carrying the HBx gene. RT-PCR was used to measure the mRNA expression of HBx at different time points. MPC5 cells were divided into 4 groups: control group (MPC5 cells cultured under normal conditions), INF-α group (MPC5 cells cultured with INF-α), HBx group (MPC5 cells induced by HBx), and HBx+INF-α group (MPC5 cells induced by HBx and cultured with INF-α). After 48 hours of intervention under different experimental conditions, flow cytometry was used to measure the apoptosis of MPC5 cells, and quantitative real-time PCR and Western blot were used to measure the mRNA and protein expression of slit diaphragm-related proteins (nephrin, CD2AP, and synaptopodin) and the cytoskeleton-related protein transient receptor potential cation channel 6 (TRPC6).
RESULTS:MPC5 cells transfected by pEX-HBx had the highest expression of HBx mRNA at 48 hours after transfection (P<0.05). Compared with the control, INF-α and HBx+INF-α groups, the HBx group had a significant increase in the apoptosis rate of MPC5 cells (P<0.05). Compared with the control and INF-α groups, the HBx group had significant reductions in the mRNA and protein expression of nephrin, synaptopodin, and CD2AP and significant increases in the mRNA and protein expression of TRPC6 (P<0.05). Compared with the HBx group, the HBx+INF-α group had significant increases in the mRNA and protein expression of nephrin, synaptopodin, and CD2AP and significant reductions in the mRNA and protein expression of TRPC6 (P<0.05).
CONCLUSIONS:INF-α can inhibit the apoptosis of podocytes induced by HBx, possibly through improving the abnormal expression of slit diaphragm-related proteins (CD2AP, nephrin, and synaptopodin) and cytoskeleton-related protein (TRPC6) induced by HBx.
