Expression of Nod-like receptor protein 3 inflammasome in peripheral blood mononuclear cells of children with Kawasaki disease in the acute stage.
- Author:
Li-Ping LIU
1
;
Yong-Hua YUAN
;
Xue-Hua HE
;
Min CHEN
;
Dan-Xia PENG
;
Wei XU
;
Xiao-Hui XIA
;
You-De CAO
;
Sheng WANG
;
Qian-Li ZHU
Author Information
1. Department of Pediatric Cardiology, Hunan People's Hospital/First Affiliated Hospital of Hunan Normal University, Changsha 410005, China. he_xh101@163.com.
- Publication Type:Journal Article
- MeSH:
Child;
Humans;
Inflammasomes;
Interleukin-1beta;
Leukocytes, Mononuclear;
Mucocutaneous Lymph Node Syndrome;
NLR Family, Pyrin Domain-Containing 3 Protein;
metabolism
- From:
Chinese Journal of Contemporary Pediatrics
2019;21(10):992-997
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the association of Nod-like receptor protein 3 (NLRP3) inflammasome with inflammatory response in the acute stage and coronary artery lesion (CAL) in children with Kawasaki disease (KD).
METHODS:A total of 42 children with KD who were hospitalized from January to October 2017 were enrolled as the KD group, among whom 9 had CAL (CAL group) and 33 had no CAL (NCAL group). Fifteen age- and gender-matched children with pneumonia and pyrexia were enrolled as the pneumonia-pyrexia group. Fifteen healthy children were enrolled as the healthy control group. Real-time PCR was used to measure the mRNA expression of NLRP3 inflammasome (NLRP3, ASC and caspase-1) in peripheral blood mononuclear cells. The Spearman rank correlation test was used to investigate the correlation of NLRP3 mRNA expression with serum levels of C-reactive protein, erythrocyte sedimentation rate, interleukin-6, interleukin-1β, procalcitonin, albumin and prealbumin.
RESULTS:The KD group had significantly higher mRNA expression of NLRP3, ASC and caspase-1 in the acute stage than the pneumonia-pyrexia and healthy control groups (P<0.05). The CAL group had significantly higher mRNA expression of NLRP3 than the NCAL group (P<0.05). NLRP3 mRNA expression was correlated with C-reactive protein, interleukin-6, interleukin-1β, and prealbumin levels in children with KD in the acute stage (r=0.449, 0.376, 0.427, and -0.416 respectively; P<0.05).
CONCLUSIONS:NLRP3 inflammasome may participate in inflammatory response in the acute stage and the development of CAL in children with KD.
