Effect of PR-957 on the formation of A1 reactive astrocytes.
- Author:
Shu-Xin DAI
1
;
Yu WANG
;
Li-Fang LIN
;
Tian-Ming YUAN
Author Information
1. Department of Neonatology, Children's Hospital of Zhejiang University, Hangzhou 310003, China. yuantianming@zju.edu.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Astrocytes;
Female;
Lipopolysaccharides;
Oligopeptides;
Rats;
Tumor Necrosis Factor-alpha
- From:
Chinese Journal of Contemporary Pediatrics
2019;21(11):1110-1115
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect of PR-957 on the formation of A1 reactive astrocytes.
METHODS:The cerebral cortices of 1-day-old female rats were obtained and cultured for primary astrocytes. These cells were divided into 3 groups: control, lipopolysaccharide (LPS), and LPS+PR-957. The LPS group was treated with LPS (at a concentration of 5 μmol/L) for 48 hours; the LPS+PR-957 group was treated with PR-957 (at a final concentration of 200 nmol/L) for 1 hour and then LPS for 48 hours. Enzyme-linked immunosorbent assay was used to determine the expression of complement 3 (C3, a marker for A1 reactive astrocytes) and tumor necrosis factor alpha (TNF-α). Quantitative real-time PCR was used to determine the relative mRNA expression of glypican-6 (GPC6), SPARC-like 1 (SPARCL1), and lipocalin-2 (LCN2). All the above experiments were repeated three times independently.
RESULTS:C3 expression was almost not observed in the control group, but was observed in both the LPS group and the LPS+PR-957 group, with significantly lower expression observed in the LPS+PR-957 group (P<0.05). The expression of TNF-α was consistent with that of C3. Compared with the control group, the LPS and the PS+PR-957 groups had significantly reduced mRNA expression levels of GPC6 and SPARCL1 but significantly increased mRNA expression level of LCN2 (P<0.001). Compared with the LPS group, the LPS+PR-957 group had significantly increased mRNA expression levels of GPC6 and SPARCL1 but significantly reduced mRNA expression level of LCN2 (P<0.001).
CONCLUSIONS:LPS can induce the transformation from astrocytes to A1 reactive astrocytes, and PR-957 can inhibit the formation of LPS-induced A1 reactive astrocytes.
