Cembrane-type diterpenoids from the South China Sea soft coral .
10.1016/j.apsb.2018.06.004
- Author:
Songwei LI
1
;
Fei YE
1
;
Zhengdan ZHU
1
;
Hui HUANG
2
;
Shuichun MAO
3
;
Yuewei GUO
1
Author Information
1. State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
2. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
3. School of Pharmacy, Nanchang University, Nanchang 330006, China.
- Publication Type:Journal Article
- Keywords:
Cembrane-type diterpe-noids;
Cytotoxicity;
ECD calculation;
Modified Mosher׳s method;
NF-κB inhibitory activity;
Sarcophyton;
Sarcophyton mililatensis;
Soft coral
- From:
Acta Pharmaceutica Sinica B
2018;8(6):944-955
- CountryChina
- Language:English
-
Abstract:
Eight cembrane-type diterpenoids, namely, (+)-(6)-6-hydroxyisosarcophytoxide (), (+)-(6)-6-acetoxyisosarcophytoxide (), (+)-17-hydroxyisosarcophytoxide (), sarcomililatins A-D (-), and sarcomililatol (), were isolated from the soft coral collected from Weizhou Island, Guangxi Autonomous Region, together with 2 known related analogues, (+)-isosarcophytoxide () and (+)-isosarcophine (). The structures of these compounds were elucidated by a combination of detailed spectroscopic analyses, chemical methods, and comparison with reported data. The absolute configuration of compound was established by the modified Mosher׳s method, while the absolute configurations of compounds and were assigned by electronic circular dichroism (ECD) spectroscopy and that of compound was established by time-dependent density functional theory electronic circular dichroism (TD-DFT ECD) calculation. In bioassays, compound displayed significant cytotoxicity against the human cancer cell lines human promyelocytic leukemia cells (HL-60) and human lung adenocarcinoma cells (A-549) with IC values of 0.78±0.21 and 1.26±0.80 μmol/L, respectively. Compounds and also showed moderate inhibitory effects on the TNF-induced Nuclear factor kappa B (NF-B, a therapeutical target in cancer) activation, showing IC values of 35.23±12.42 and 22.52±4.44 μmol/L, respectively.
