Novel fluorescent probes of 10-hydroxyevodiamine: autophagy and apoptosis-inducing anticancer mechanisms.
10.1016/j.apsb.2018.08.003
- Author:
Shuqiang CHEN
1
;
Guoqiang DONG
1
;
Shanchao WU
1
;
Na LIU
1
;
Wannian ZHANG
1
;
Chunquan SHENG
1
Author Information
1. School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
- Publication Type:Journal Article
- Keywords:
10-Hydroxyevodiamine;
3MA, 3-methyladenine;
Anticancer mechanisms;
Apoptosis;
Autophagy;
Boc, di-tert-butyl dicarbonate;
CCK8, cell counting kit-8;
DMAP, 4-dimethylaminopyridine;
DMSO, dimethylsulfoxide;
EDC, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride;
Fluorescent probes;
HBTU, O-benzotriazole-N,N,N,N-tetramethyl-uronium-hexafluorophosphate;
MMP, mitochondrial membrane potential;
NPs, natural products;
TEA, trimethylamine;
TFA, trifluoroacetic acid
- From:
Acta Pharmaceutica Sinica B
2019;9(1):144-156
- CountryChina
- Language:English
-
Abstract:
Natural product evodiamine and its derivatives represent a promising class of multi-target antitumor agents. However, the clinical development of these compounds has been hampered by a poor understanding of their antitumor mechanisms. To tackle this obstacle, herein, novel fluorescent probes were designed to elucidate the antitumor mode of action of 10-hydroxyevodiamine. This compound was proven to be distributed in the mitochondria and lysosomes and to act by autophagy and apoptosis mechanisms.
