Novel C-17 spirost protostane-type triterpenoids from  with anti-inflammatory activity in Caco-2 cells.

Author: Qinghao JIN ¹ ; Jianqing ZHANG ¹ ; Jinjun HOU ¹ ; Min LEI ¹ ; Chen LIU ¹ ; Xia WANG ¹ ; Yong HUANG ¹ ; Shuai YAO ¹ ; Bang Yeon HWANG ² ; Wanying WU ¹ ; Dean GUO ¹
Author Information

1. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
2. College of Pharmacy, Chungbuk National University, Cheongju 2816, South Korea.
Publication Type:Journal Article
Keywords: Alisma plantago-aquatica Linn.; Caco-2 cells; LPS-induced NO production; Protostane-type triterpenoids
From: Acta Pharmaceutica Sinica B 2019;9(4):809-818
CountryChina
Language:English
Abstract: Twenty-one protostane-type triterpenoids with diverse structures, including nine new compounds (-), were isolated from the of Linn. Structurally, alisolides A‒F (-), composed of an oxole group coupled to a five-membered ring, represent unusual C-17 spirost protostane-type triterpenoids. Alisolide H () is a novel triterpenoid with an unreported endoperoxide bridge. Alisolide I () represents the first example of 23,24-acetal triterpenoid. Their structures were elucidated based on spectroscopic analysis, wherein the absolute configurations of ‒, were further confirmed by the Mo(OAc)-induced ECD method. Furthermore, all isolates were evaluated for their inhibitory effects on LPS-induced NO production in Caco-2 cells, and all the compounds showed remarkable inhibitory activities, with IC values in the range of 0.76-38.20 μmol/L.