Anti-inflammatory Effect of Dactyloquinone B and Cyclospongiaquinone-1 Mixture in RAW264.7 Macrophage and ICR Mice.
10.20307/nps.2015.21.4.268
- Author:
Dong Sung LEE
1
;
In Hyun HWANG
;
Nam Kyung IM
;
Gil Saeng JEONG
;
Minkyun NA
Author Information
1. Department of Biomedical Chemistry, Konkuk University, Chungju 380-701, Korea.
- Publication Type:Original Article
- Keywords:
Dactyloquinone B;
Cyclospongiaquinone-1;
Anti-inflammatory effect;
Ear edema model
- MeSH:
Animals;
Cyclooxygenase 2;
Ear;
Edema;
Interleukin-1beta;
Interleukin-6;
Macrophages*;
Mice;
Mice, Inbred ICR*;
Nitric Oxide;
Nitric Oxide Synthase Type II;
Porifera;
Tumor Necrosis Factor-alpha
- From:Natural Product Sciences
2015;21(4):268-272
- CountryRepublic of Korea
- Language:English
-
Abstract:
Sesquiterpene-quinone is a class of secondary metabolites frequently encountered from marine sponge. The present study was designed to examine the anti-inflammatory action of sponge-derived dactyloquinone B (DQB) and cyclospongiaquinone-1 (CSQ1) mixture using lipopolysaccharide (LPS)-induced inflammatory responses. We measured the production of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6) and expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein. TNF-alpha, IL-1beta, and IL-6 production, which increased by treatment with LPS, were significantly inhibited by DQB and CSQ1 mixture. It also decreased the production of NO production, and iNOS and COX-2 expression. Furthermore, it reduced 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced ear edema of ICR mice. These results demonstrate that sesquiterpene-quinone, DQB and CSQ1 mixture, might serve as a chemical pipeline for the development of anti-inflammatory agent.
