Study on proteins in Guangdilong by nano LC/orbitrap fusion lumos HR-MS.
10.19540/j.cnki.cjcmm.20181009.012
- Author:
Hong-Shuang DONG
1
;
Jing-Xian ZHANG
2
;
Qing HU
2
;
Yan-Chun WANG
3
;
Jian SUN
2
;
Su ZHANG
2
;
Hong YU
2
;
Rui FENG
2
;
Xiu-Hong MAO
2
;
Shen JI
1
Author Information
1. Shanghai Institute for Food and Drug Control Shanghai 201203,China Shanghai Institute of Pharmaceutical Industry Shanghai 201203,China.
2. Shanghai Institute for Food and Drug Control Shanghai 201203,China.
3. Shanghai Kangqiao Chinese Medicine Tablet Co.,Ltd. Shanghai 201315,China.
- Publication Type:Journal Article
- Keywords:
Guangdilong;
SDS-PAGE;
orbitrap fusion lumos;
proteome
- MeSH:
Animals;
Chromatography, Liquid;
Electrophoresis, Polyacrylamide Gel;
Gene Ontology;
Mass Spectrometry;
Oligochaeta;
chemistry;
Proteome;
Proteomics
- From:
China Journal of Chinese Materia Medica
2019;44(2):324-331
- CountryChina
- Language:Chinese
-
Abstract:
In the present study,fresh Guangdilong( GD),originating from Pheretima aspergillum,was taken as the object. The total proteins from GD were firstly separated by SDS-PAGE according to their molecular weights and in-gel digestion was then performed.After that,the peptides were analyzed by nano LC/orbitrap fusion lumos high resolution mass spectrometry( nano LC/orbitrap fusion lumos HR-MS). Protein identification was implemented by comparison with Annelida. fasta database using Proteome Discoverer software.As a result,386 proteins were tentatively identified,including chain F,globin B chain,glyceraldehyde-3-phosphate dehydrogenase,fibrinolytic protein,and so on. Most of the proteins took part in cell structure and energy metabolism,and fibrinolytic protein and lombricine kinase might be related to fibrinolytic activity. Protein classification based on gene ontology was carried out using PANTHER and KEGG for metabolic pathway enrichment. The results indicated that these proteins were related to diverse signal transduction pathways,including metabolic pathways,central carbon metabolism,biosynthesis of amino acids,ribosome,glycolysis,citrate cycle( TCA cycle),and so on. This study would lay the foundation for the further research on the proteins in GD and also their functions.
