Comparative analysis on chemical constituents in Bupleurum chinense,B. marginatum,B. marginatum var. stenophyllum and B. smithii var. parvifolium.
10.19540/j.cnki.cjcmm.20181009.010
- Author:
Yin-Jun YANG
1
;
Wei ZHENG
2
;
Jia-Qi GUO
3
;
Wei FANG
3
;
Bai-Ping MA
2
;
Bao-Lin GUO
3
;
Yun-Xia PENG
4
;
Dong-Jia ZHANG
4
Author Information
1. Key Laboratory of Bioactive Substance and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193,China Institute of Radiation Medicine,Academy of Military Medical Sciences Beijing 100850,China.
2. Institute of Radiation Medicine,Academy of Military Medical Sciences Beijing 100850,China.
3. Key Laboratory of Bioactive Substance and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193,China.
4. Institute of Chinese Herbal Medicines,Gansu Academy of Agricultural Sciences Lanzhou 730070,China.
- Publication Type:Journal Article
- Keywords:
Bupleurum;
PCA;
UPLC-Q-TOF-MS~E;
component identification
- MeSH:
Bupleurum;
chemistry;
classification;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
Phytochemicals;
analysis;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2019;44(2):332-337
- CountryChina
- Language:Chinese
-
Abstract:
UPLC-Q-TOF-MS was used to analyze the chemical differences in Bupleurum. chinense,B. marginatum,B. marginatum var. stenophyllum and B. smithii var. parvifolium. Chromatographic separation was carried out on an Acquity HSS T3 C_(18) column( 2. 1 mm ×100 mm,1. 8 μm,Waters) with the mobile phase composed of 0. 1% formic acid in water-acetonitrile in the gradient elution. A hybrid quadrupole time-of-flight tandem mass spectrometry( Q-TOF-MS~E) was used for mass spectrometric analysis. Finally,25 peaks were identified based on their exact mass data and fragmentation characteristics. B.chinense,B.marginatum,B. marginatum var. stenophyllum and B. smithii var. parvifolium were obviously clustered into 3 types through processing by principal component analysis( PCA). There was almost no difference between B. chinense and B. marginatum. However,the compounds existed in B. chinense were different from those in B. marginatum var. stenophyllum,and B. smithii var. parvifolium.
