Study on wavelength overlapping HPLC fingerprint and multi-component content determination of Danshen.
10.19540/j.cnki.cjcmm.20181009.011
- Author:
Tao WANG
1
;
Hui ZHANG
2
;
Dan ZHAO
1
;
Jie CHEN
1
;
Zhuo-Lin XIE
1
;
Wei DAI
1
;
Kun-Hao YE
1
;
Jing HUANG
3
;
Gang-Fu CHEN
4
Author Information
1. Institute of Chinese Medicinal Materials,Mianyang Academy of Agricultural Sciences Mianyang 621023,China Comprehensive Experimental Station of Mianyang,Chinese Materia Medica of China Agriculture Research System Mianyang 621023,China.
2. Biotechnology Center,Mianyang Academy of Agricultural Sciences Mianyang 621023,China.
3. School of Life and Engineering,Southwest University of Science and Technology Mianyang 621010,China.
4. Mianyang Dadi Maidong Industrial Technology Research Institute Mianyang 621023,China Sichuan Daidaiweiben Agricultural Science & Technology Co. Mianyang 621100,China.
- Publication Type:Journal Article
- Keywords:
Danshen;
HPLC;
fingerprint;
multi-component content;
wavelength overlapping
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
analysis;
Phytochemicals;
analysis;
Quality Control;
Salvia miltiorrhiza;
chemistry
- From:
China Journal of Chinese Materia Medica
2019;44(2):338-343
- CountryChina
- Language:Chinese
-
Abstract:
This project was launched to and establish the wavelength overlapping HPLC fingerprint for Danshen and determine the contents of 7 component in Danshen. The chromatographic fingerprints were built by using Agilent Eclipse Plus C_(18)( 4. 6 mm×100 mm,3. 5 μm) as stationary phase and 0. 1% formic acid solution( A)-acetonitrile( B) as mobile phase with gradient elution( 0-5 min,10%-20% B; 5-20 min,20%-30% B; 20-25 min,30%-50% B; 25-40 min,50%-65% B; 40-45 min,65%-80% B; 45-46 min,80%-10% B; 46-50 min,10% B) at a flow rate of 1 mL·min~(-1). The column temperature was maintained at 30 ℃ and the detection wavelength was set at 250,280,310 and 340 nm. The technique of wavelength overlapping fingerprint was established and the contents of 7 indicative compounds have been determined in this method. The results of wavelength overlapping HPLC fingerprint showed all-around information of the fingerprints at 250,280,310 and 340 nm,and the similarity among 17 batches of Danshen was over 0. 828-0. 936. In wavelength overlapping HPLC fingerprint,15 common peaks were selected as the common peaks,and 7 contents of them were indentified as rosmarinic acid,salvianolic acid B,salvianolic acid A,phenolic acid,dihydrotanshinone Ⅰ,tanshinone Ⅰ and tanshinone ⅡA. The results of methodological study demonstrated that the method met the requirements of the determination. The method established in this study is simple,convenient and durable,which can provide a scientific basis for the quality control of Danshen.
