Diversity and difference of endophytes in Dendrobium huoshanense with different growth years.
10.19540/j.cnki.cjcmm.2019.0022
- Author:
Shao-Tong CHEN
1
;
Jun DAI
2
;
Xue-Ping JIANG
2
;
Xiang-Wen SONG
2
;
Cun-Wu CHEN
2
;
Nai-Fu CHEN
2
;
Lan-Ping GUO
3
;
Bang-Xing HAN
2
Author Information
1. College of Pharmacy,Anhui University of Chinese Medicine Hefei 230038,China College of Biological and Pharmaceutical Engineering,West Anhui University Lu'an 237012,China.
2. College of Biological and Pharmaceutical Engineering,West Anhui University Lu'an 237012,China Anhui Province Traditional Chinese Medicine Resource Protection and Sustainable Utilization Engineering Laboratory Lu'an 237012,China.
3. National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Science Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
Dendrobium huoshanense;
endophytes;
growing year;
metagenomics
- MeSH:
Ascomycota;
Bacteria;
Dendrobium;
Endophytes;
Fungi;
Fusarium;
Phylogeny
- From:
China Journal of Chinese Materia Medica
2019;44(6):1145-1150
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore endophytes diversity and difference in Dendrobium huoshanense,in this paper,the metagenomics method was used to analyze the endophytic bacteria and fungi community of 5 groups include 30 samples in different growth years. The results indicate that 3 540 bacterial OTUs were identified from D. huoshanense,and there are 138 OTUs in 5 groups simultaneously;2 168 fungal OTUs were identified,and 143 OTUs exist in 5 groups simultaneously. The dominate endophytic bacteria community are Sphingomonas sp.,Acinetobacter sp.,Burkholderia sp.,Methylobacterium sp.,Enterococcus sp.,Bacillus sp.,the difference endophytic bacteria community are Oceanobacillusd sp.,Actinomycetospora sp.,Paenibacillus sp.. The dominate endophytic fungi community are Zasmidium sp.,Zymoseptoria sp.,Alternaria sp.,Cladosporium sp.,Fusarium sp.,the difference endophytic fungi community are Cyphellophore sp.,Fusarium sp.. The results of clustering revealed that both the endophytic bacteria and the endophytic fungi,ⅢY2 and ⅢY3 are complete clustered,and ⅡY1 and ⅢY1 are also cluster completely. These enriched the species and resources of endophytic bacteria and fungi in D. huoshanense,and provided a theoretical reference for the reasonable harvest of D. huoshanense.
