Effects of Euphorbiae Pekinensis Radix before and after processing with vinegar on liver and gastrointestinal toxicity of zebrafish embryos.
10.19540/j.cnki.cjcmm.20181220.004
- Author:
Fang YAO
1
;
Kai-Cheng ZHANG
1
;
Yu-Dan CAO
1
;
Li ZHANG
1
Author Information
1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine Nanjing 210023,China.
- Publication Type:Journal Article
- Keywords:
Euphorbiae Pekinensis Radix;
liver and gastrointestinal toxicity;
vinegar-process;
zebrafish embryos
- MeSH:
Acetic Acid;
Animals;
Drugs, Chinese Herbal;
Liver;
Plant Roots;
Zebrafish
- From:
China Journal of Chinese Materia Medica
2019;44(6):1179-1185
- CountryChina
- Language:Chinese
-
Abstract:
To study the effects of different fraction of Euphorbiae Pekinensis Radix before and after processing with vinegar on liver and gastrointestinal toxicity of zebrafish embryos,the zebrafish embryos after fertilized 12 h(12 hpf) were exposed to different concentrations of solution until 96 h(96 hpf),for observation of the toxicity response of the liver and gastrointestinal of individual zebrafish embryos. The results showed that toxicity increased in a dose-dependent manner. The liver and gastrointestinal toxicity of the zebrafish embryos in various polar fractions of Euphorbiae Pekinensis Radix before and after processing with vinegar was mainly manifested as slow liver development,smaller liver area,edema of yolk sac,delayed absorption,slowing of gastrointestinal motility,abnormal function of gastrointestinal goblet cell secretion. In addition,the toxicity of different polarity was followed by petroleum ether,dichloromethane,ethyl acetate. The above results indicated that the toxicity was reduced after processing with vinegar,and the fractions of petroleum ether and methylene chloride were the main sites responsible for liver and gastrointestinal toxicity.
