Protective effects of flavonoids from Gynostemma pentaphyllum on oxidative damage in LLC-PK1 cells.
10.19540/j.cnki.cjcmm.20181226.019
- Author:
Man LIN
1
;
Yu-Rong WANG
1
;
Xin-Fang ZHAI
1
;
Shao-Fang XING
1
;
Xiang-Lan PIAO
1
Author Information
1. School of Pharmacy,Minzu University of China Beijing 100081,China.
- Publication Type:Journal Article
- Keywords:
Gynostemma pentaphyllum;
LLC-PK1 cells;
flavonoids;
oxidative damage
- MeSH:
Animals;
Antioxidants;
Flavonoids;
Gynostemma;
LLC-PK1 Cells;
Oxidative Stress;
Plant Extracts;
Quercetin;
Swine
- From:
China Journal of Chinese Materia Medica
2019;44(6):1193-1200
- CountryChina
- Language:Chinese
-
Abstract:
Four flavonoids were isolated from Gynostemma pentaphyllum by chromatography methods and their structures were identified by MS and NMR spectra data as quercetin-3-O-( 2″,6″-di-α-L-rhamnosyl)-β-D-galactopyranoside( 1),quercetin-3-O-( 2″,6″-di-α-L-rhamnosyl)-β-D-glucopyranoside( 2),quercetin-3-O-( 2″-α-L-rhamnosyl)-β-D-galactopyranoside( 3),and quercetin-3-O-( 2″-α-L-rhamnosyl)-β-D-glucopyranoside( 4). Among them,compounds 1-3 were obtained from the Cucurbitaceae family for the first time.The four flavonoids showed potent antioxidant effects against the DPPH,·OH and ■radicals in vitro,especially for DPPH radical scavenging activity with the IC50 values of 71. 4,29. 5,48. 3 and 79. 2 μmol·L~(-1),respectively. Moreover,the four flavonoids displayed strong cytoprotection against AAPH-induced oxidative damage in LLC-PK1 cells by suppressing the increase of malondialdehyde( MDA) and the decrease of the superoxide dismutase( SOD) and glutathione( GSH). Since further research is needed to prove its efficacy in vivo and clinical trial,the study may provide four potential antioxidants from G. pentaphyllum.
