Study on preparation of volatile oil from Acorus tatarinowii self-nanoemulsion dropping pills and its protective effect on acute myocardial ischemia injury.
10.19540/j.cnki.cjcmm.20181220.006
- Author:
Rui-Na ZHONG
1
;
Xiao-Han WANG
1
;
Lu WAN
2
;
Cheng-Ying SHEN
1
;
Bao-de SHEN
3
;
Jing WANG
1
;
Li HAN
4
;
Hai-Long YUAN
3
Author Information
1. Department of Pharmacy,Air Force Specialty Medical Center Beijing 100142, China College of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
2. Academic Affairs Office(Experimental Management Center), Science and Technology of Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.
3. Department of Pharmacy,Air Force Specialty Medical Center Beijing 100142, China.
4. College of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
- Publication Type:Journal Article
- Keywords:
acute myocardial ischemia;
in vitro dissolution;
orthogonal design;
volatile oil from Acorus tatarinowii self-nanoemulsion dropping pills
- MeSH:
Acorus;
chemistry;
Animals;
Creatine Kinase;
blood;
Drugs, Chinese Herbal;
pharmacology;
Malondialdehyde;
blood;
Myocardial Ischemia;
drug therapy;
Oils, Volatile;
pharmacology;
Plant Oils;
pharmacology;
Rats;
Superoxide Dismutase;
blood
- From:
China Journal of Chinese Materia Medica
2019;44(7):1357-1362
- CountryChina
- Language:Chinese
-
Abstract:
In this study, solid dispersion technology was used to develop volatile oil from Acorus tatarinowii self-nanoemulsion dropping pills(VOA-SNEDDS-DP) and its protective effect on acute myocardial ischemia injury was evaluated. Taking exterior quality, weight variation and the resolving time as comprehendsive evaluation indexes, the preparation process and formulation of the dropping pills were optimized by orthogonal design, and the dissolution rate in vitro of the optimized VOA-SNEDDS-DP was investigated. The rat model of acute myocardial ischemia was induced by intraperitoneal injection of isoproterenol hydrochloride and the serum levels of superoxide dismutase(SOD), malondialdehyde(MDA), creatine kinase(CK) and pathological changes of myocardial tissue were determined to evaluate therapeutic effect of the dropping pills on acute myocardial ischemia. The results showed that the optimal formulation and preparation process of VOA-SNEDDS-DP were as follows: PEG6000-PEG8000 was 1∶1, proportion of VOA-SNEDDS and matrix was l∶2.5, the temperature of drug fluids was 75 ℃, drop rate was 35 drops/min, drop distance was 5 cm, the condensing agent temperature was 2-10 ℃. The content of β-asarone in the dropping pills was 42.46 mg·g~(-1). The accumulated dissolution rate of the dropping pills reached 93.85% in 10 min. The results of pharmacodynamic experiments showed that VOA-SNEDDS-DP could significantly increase the SOD content(P<0.05), reduce the levels of MDA and CK(P<0.05) in serum, and effectively improve the pathological morphology of myocardial tissue. These results revealed that the preparation of VOA-SNEDDS-DP by solid dispersion technology was stable and feasible, and VOA-SNEDDS-DP had protective effect on acute myocardial ischemia injury.
