Quality evaluation of Poria based on specific chromatogram and quantitative analysis of multicomponents.
10.19540/j.cnki.cjcmm.20181220.005
- Author:
Shuang-Shuang TIAN
1
;
Xiao-Qian LIU
2
;
Wei-Hong FENG
2
;
Qi-Wei ZHANG
2
;
Li-Hua YAN
2
;
Zhi-Min WANG
2
;
Lu GAO
3
Author Information
1. Henan University of Chinese Medicine Zhengzhou 450000, China National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Xiuzheng Pharmaceutical Group Co., Ltd. Tonghua 134000, China.
- Publication Type:Journal Article
- Keywords:
HPLC;
Poria cocos;
contents determination;
specific chromatograms;
triterpenoids
- MeSH:
China;
Chromatography, High Pressure Liquid;
Materia Medica;
analysis;
Poria;
chemistry
- From:
China Journal of Chinese Materia Medica
2019;44(7):1371-1380
- CountryChina
- Language:Chinese
-
Abstract:
HPLC specific chromatograms of Poria were established, and the concentrations of 10 triterpenoids(16α-hydroxydehydrotrametenolic acid, poricoic acid B, dehydrotumulosic acid, poricoic acid A, polyporenic acid C, poricoic acid AM, 3-O-acetyl-16α-hydroxydehydrotrametenolic acid, dehydropachymic acid, pachymic acid, and dehydrotrametenolic acid) were simultaneously determined. Chromatographic analysis was conducted on a Welch Ultimate XB C_(18) column(4.6 mm × 250 mm,5 μm). Acetonitrile solution(contain 3% tetrahydrofuran)(A) and 0.1% formic acid aqueous solution(B) were used as the mobile phase with gradient elution at a flow rate of 1.0 mL·min~(-1). The column temperature was 30 ℃ and the injection volume was 20 μL. The experimental data were analyzed by the SPSS 22.0 and GraphPad Prism 7.0. The established triterpenoids fingerprints were specific, and the 10 components were well separated and showed good linearity(r≥0.999 6) within the concentration ranges tested. The mean recoveries were between 98.53%-103.8%(RSD 1.7%-2.7%). The method was specific and repeatable, and could be used for identification and quality evaluation of Poria. The results showed that the contents of 10 triterpenoids were positively correlated with each other. The contents of 10 triterpenoids of samples collected from producing areas were higher than that collected from markets. The total contents of 10 triterpenoids of samples collected from Hubei and Yunnan province were slightly higher than that from Anhui province, but the contents of samples from Anhui province were varied in smaller ranges.
