Simultaneous determination of 8 bioactive isoflavonoids in Astragali Radix by UHPLC equipped with core-shell column.
10.19540/j.cnki.cjcmm.20190118.006
- Author:
Dan TANG
1
;
Dong-Min CAO
1
;
Lan-Fang TAN
1
;
You-Hua XU
2
;
Ting-Ting DUAN
3
;
Quan ZHU
4
;
Shu-Mei WANG
1
Author Information
1. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of SATCM Guangzhou 510006, China Engineering Technology Research Center for Chinese Materia Medica Quality of Guangdong Province Guangzhou 510006, China Engineering & Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province Guangzhou 510006, China.
2. State Key Laboratory of Quality Research in Chinese Medicine, Faculty of Chinese Medicine,Macau University of Science and Technology Macau 999078, China.
3. Institute of Consun Co.for Chinese Medicine in Kidney Diseases,Guangdong Consun Pharmaceutical Group Guangzhou 510530, China.
4. State Key Laboratory of Quality Research in Chinese Medicine, Faculty of Chinese Medicine,Macau University of Science and Technology Macau 999078, China Institute of Consun Co.for Chinese Medicine in Kidney Diseases,Guangdong Consun Pharmaceutical Group Guangzhou 510530, China.
- Publication Type:Journal Article
- Keywords:
Astragali Radix;
core-shell column;
isoflavonoids;
quantitative evaluation
- MeSH:
Astragalus Plant;
chemistry;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
standards;
Flavones;
analysis;
Phytochemicals;
analysis;
Plant Roots;
chemistry;
Quality Control
- From:
China Journal of Chinese Materia Medica
2019;44(7):1410-1415
- CountryChina
- Language:Chinese
-
Abstract:
This research aims to develop an UHPLC method, based on core-shell column(i.e. superficially porous particles), for simultaneous determination of eight isoflavonoids including formononetin,(6αR,11αR)-3-hydroxy-9,10-dimethoxypterocarpan, calycosin-7-O-β-D-glucopyranoside,(3R)-7,2-dihydroxy-3,4-dimethoxyisoflavone, calycosin, ononin,(6αR,11αR)-9,10-dimethoxypterocarpan-3-O-β-D-glucopyranoside, and(3R)-7,2-dihydroxy-3,4-dimethoxyisoflavan-7-O-β-D-glucopyranoside in Astragali Radix. The analysis was performed on an Agilent Poroshell EC-C_(18 )column(2.1 mm×100 mm, 2.7 μm) with 0.2% formic acid solution(A)-acetonitrile(B) as mobile phase for gradient elution. The flow rate was 0.5 mL·min~(-1), with column temperature of 40 ℃ and the wavelengths were set at 260 and 280 nm. According to the results, all calibration curves showed good linearity(R~2>0.999 8) within the tested concentration ranges. Both the intra-and inter-day precisions for 8 isoflavonoids were less than 0.80%, with the mean recovery at the range of 94.71%-104.6%. Thus, the newly developed UHPLC method using core-shell column owned the advantages in terms of rapid analysis, low column pressure and less solvent consumption, thus enabling the usage of conventional HPLC systems. Meanwhile, quantitative evaluation was carried out for 22 batches of commercial Astragali Radix. It has been found that great variations occurred for the content of the individual isoflavonoids among different batches; in contrast, the total content of total 8 isoflavonoids(>0.1%) was stable in most samples, indicating that it was reasonable to involve all isoflavonoids as the chemical markers for the quality control of Astragali Radix.
