Determination of plasma protein binding rates of nine compounds of Inula cappa extraction based on method of equilibrium dialysis.
10.19540/j.cnki.cjcmm.20190116.007
- Author:
Hong-Song BAO
1
;
Jing-Yu HOU
2
;
He-Jia HU
2
;
Yue-Ting LI
2
;
Lin ZHENG
2
;
Yong HUANG
2
;
Guang-Cheng WANG
2
;
Meng ZHOU
2
;
Yan-Yu LAN
2
;
Zi-Peng GONG
2
Author Information
1. School of Pharmacy, Guizhou Medical University, Provincial Key Laboratory of Pharmaceutics in Guizhou,State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine Guiyang 550004, China Department of Pharmacy, Guizhou Orthopedics Hospital Guiyang 550002, China.
2. School of Pharmacy, Guizhou Medical University, Provincial Key Laboratory of Pharmaceutics in Guizhou,State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine Guiyang 550004, China.
- Publication Type:Journal Article
- Keywords:
Inula cappa;
caffeoylquinic acid;
equilibrium dialysis;
plasma protein binding rates
- MeSH:
Animals;
Blood Proteins;
metabolism;
Chromatography, High Pressure Liquid;
Humans;
Inula;
chemistry;
Phytochemicals;
metabolism;
Plant Extracts;
metabolism;
Protein Binding;
Rats;
Reproducibility of Results;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2019;44(7):1475-1484
- CountryChina
- Language:Chinese
-
Abstract:
To determine the plasma protein binding rate of the nine compounds in Inula cappa extraction by the method of equilibrium dialysis. The proteins in plasma samples were precipitated by methanol, and the ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed for determination of the concentrations of the nine active compounds, namely chlorogenic acid, scopolin, neochlorogenic acid, cryptochlorogenic acid, 1,3-O-dicaffeoylquinic acid, galuteolin, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, with the internal standard of puerarin. We found that all components have a good linearity(r≥0.999), and accuracy, precision, extraction recovery and stability conformed to the requirements of determination, without endogenous compounds disturbing within the range of optimum concentration. This suggested that the method was stable and reliable, and could be used for the determination of the plasma protein binding rates of the nine active compounds in rat and human plasma of I. cappa. The plasma protein binding rates of the nine active compounds in rat and human plasma respectively were(41.07±0.046)%-(94.95±0.008)%, and(37.66±0.043)%-(97.46±0.013)%. According to the results, there were differences in the plasma protein binding rates of the nine compounds in I. cappa extraction between rat and human.
