Cloning and quantitative expression analysis of GMPP gene from Dendrobium huoshanense.
10.19540/j.cnki.cjcmm.20190129.013
- Author:
Rong-Chun HAN
1
;
Lu-Lu LIU
1
;
Jun-Lin LIU
1
;
Dong-Mei XIE
1
;
Dai-Yin PENG
1
;
Nian-Jun YU
1
Author Information
1. Institute of Traditional Chinese Medicine Resources Protection and Development,Anhui Academy of Chinese Medicine,Anhui University of Chinese Medicine Hefei 230012,China.
- Publication Type:Journal Article
- Keywords:
Dendrobium huoshanense;
GMPP;
gene expression;
qPCR
- MeSH:
Base Sequence;
Cloning, Molecular;
Dendrobium;
enzymology;
genetics;
Nucleotidyltransferases;
genetics;
Phylogeny;
Plant Proteins;
genetics;
Polysaccharides;
biosynthesis
- From:
China Journal of Chinese Materia Medica
2019;44(8):1552-1557
- CountryChina
- Language:Chinese
-
Abstract:
In order to understand the function of GDP-mannose pyrophosphorylase(GMPP) function and its regulation in polysaccharide biosynthesis mechanism in Dendrobium. D. huoshanense was used to clone GMPP gene. GMPP gene expression in D. huoshanense,D. officinale and D. moniliforme was also determined by qPCR. The results showed that the length of D. huoshanense GMPP gene c DNA sequence is 1 867 bp,containing 1 245 bp open reading frame(ORF),encoding 415 amino acids. Phylogenetic tree analysis showed that D. huoshanense,D. officinale and D. moniliforme are closely related with GMPP taken into consideration. Bioinformatics analysis demonstrated that GMPP sequence similarity among the three species reached as high as 99%. qPCR results indicated that GMPP genes was highly expressed in stem of D. huoshanense compared with its leaf,flower and root. According to GMPP gene expression profile in D. huoshanense,D. officinale and D. moniliforme grown in Huoshan area,it was clear that GMPP in D. huoshanense showed the highest expression level. Furthermore,our findings of GMPP gene expression profile will facilitate future researches into its polysaccharide biosynthetic mechanism.
