Characteristic fingerprint and multi-components quantitative determination for Fuke Qianjin Capsules by HPLC.
10.19540/j.cnki.cjcmm.20181220.007
- Author:
Kang-Hua WANG
1
;
Ying-Tao ZHANG
1
;
Xiu-Wei YANG
1
;
Wei XU
1
;
Peng ZHANG
2
;
Kai-Feng PENG
2
;
Fu-Jun LI
2
;
Yun GONG
2
;
Ni-Fu LIU
2
Author Information
1. State Key Laboratory of Natural and Biomimetic Drugs,Department of Natural Medicines,School of Pharmaceutical Sciences,Peking University Beijing 100191,China.
2. Zhuzhou Qianjin Pharmaceutical Co.,Ltd. Zhuzhou 412000,China.
- Publication Type:Journal Article
- Keywords:
14-deoxy-11,12-didehydroandrographolide;
Fuke Qianjin Capsules;
HPLC characteristic fingerprint;
andrographolide;
genistin;
jatrorrhizine;
multi-index quantitative analysis;
quality control
- MeSH:
Capsules;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
chemistry;
Phytochemicals;
analysis
- From:
China Journal of Chinese Materia Medica
2019;44(8):1564-1572
- CountryChina
- Language:Chinese
-
Abstract:
To establish an HPLC characteristic fingerprint method of Fuke Qianjin Capsules,and determine the contents of its main components. The analysis was carried out on a Kromasil 100-5-C18 analytical column(4. 6 mm ×250 mm,5 μm) with gradient elution by acetonitrile(A)-0. 1% phosphoric acid aqueous solution(B),a flow rate at 1. 0 m L·min-1 and the detection wavelength of 254 nm.The column temperature was 30 ℃,and the injection volume was 10 μL. The determination method of genistin,jatrorrhizine,andrographolide and 14-deoxy-11,12-didehydroandrographolide index components were studied methodologically. The common mode of the characteristic fingerprint of Fuke Qianjin Capsules was set up with 8 common peaks,which were identified as genistin,jatrorrhizine,palmatine,berberine,andrographolide,14-deoxy-11,12-didehydroandrographolide,Z-ligustilide,and Z-3-butylidenephthalide,respectively,in comparison with the references. The similarities of 20 batches of Fuke Qianjin Capsules samples were above 0. 95. All of the above-mentioned 4 analytes could be well separated under the optimized chromatographic conditions. RSD of precision and repeatability experiment were both less than 1. 5%,and the sample solution was stable during 72 h. All of the compounds had a good linearity and linear range. The contents of genistin,jatrorrhizine,andrographolide,and 14-deoxy-11,12-didehydroandrographolide in 20 batches of Fuke Qianjin Capsules samples were 28. 66-56. 04,94. 77-197. 92,1 705. 33-4 148. 93 and 462. 16-1 225. 96 μg in each capsule,respectively. The developed HPLC characteristic fingerprint and quantitative analysis methods were reliable,accurate and sensitive,and could be used effectively evaluate the quality of Fuke Qianjin Capsules samples.
