Screening of Shuanghuanglian Injection allergenic ingredients based on immune fingerprint.
10.19540/j.cnki.cjcmm.20190325.301
- Author:
Wen WEN
1
;
Fu-Yuan HE
2
;
Wen-Long LIU
2
;
Yi-Qun ZHOU
2
;
Hong-Yu LIU
3
Author Information
1. Department of Pharmaceutics,Hunan University of Chinese Medicine Changsha 410208,China First Affiliated Hospital of Hunan University of Chinese Medicine Changsha 410007,China.
2. Department of Pharmaceutics,Hunan University of Chinese Medicine Changsha 410208,China Hunan Provincial Key Laboratory of Druggability and Preparation Modification of Traditional Chinese Medrcine Changsha 410208,China.
3. First Affiliated Hospital of Hunan University of Chinese Medicine Changsha 410007,China.
- Publication Type:Journal Article
- Keywords:
Shuanghuanglian Injection;
anaphylactoid;
anti-IgE antibody;
immune fingerprint;
supramolecular
- MeSH:
Allergens;
analysis;
Animals;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
adverse effects;
chemistry;
Enzyme-Linked Immunosorbent Assay;
Hypersensitivity;
Injections;
Medicine, Chinese Traditional;
Rats
- From:
China Journal of Chinese Materia Medica
2019;44(8):1588-1595
- CountryChina
- Language:Chinese
-
Abstract:
In this paper,immune fingerprint was used to screen the allergenic components of Shuanghuanglian Injection(SHLI) by enzyme-linked immuno sorbent assay(ELISA) combined with HPLC/MS method. ELISA-embedded anti-IgE antibody could successfully adsorb allergens in SHLI and its plasma samples containing drugs through different routes of administration,suggesting that SHLI can induce type I hypersensitivity in rats. HPLC fingerprints and MS map of SHLI and drug-containing plasma samples from different routes of administration before and after anti-IgE antibody adsorption were established. According to the similarity evaluation of HPLC fingerprints and analysis results MS map,the sensitization of traditional Chinese medicine injections can be changed by different administration methods. There were 22 kinds of components that can be adsorbed by specific anti-Ig E antibodies in Shuanghuanglian Injection and its drug-containing plasma,most of them were acids and nitrogen compounds. Based on supramolecular theory,it was inferred that these compounds came from SHLI or body,and may form supramolecular hapten,which results in immunotoxicity and allergic reaction when being used as injection instead of oral liquid. Immune fingerprint is not only used to screen out single component allergen,but also more comprehensive,sensitive and easy to operate. It can provide reference for the future research methods of allergic reaction of traditional Chinese medicine injections.
